摘要
[目的]探讨他汀类(statins)药物S imvastatin促进大鼠坐骨神经修复及其免疫调节机制。[方法]制作SD大鼠坐骨神经钳夹损伤(crush)模型,分别予S imvastatin和溶媒(0.3%羧甲基纤维素钠)对照干预2周,并设立假手术组。手术后作行为学、神经电生理学、组织学计价和血清TNFα-和IL-6检测。[结果]S imvastatin干预组趾展功能指数在术后5、8 d较对照组大,2周肌肉复合动作电位(CMAP)幅度高,4周神经传导速度(NCV)快;手术后5 d,血清IL-6和TNFα-水平均低于对照组,尤以IL-6为明显;S imvastatin干预组神经再生形态优于对照组。[结论]S imvastain可能通过减少血清IL-6和TNFα-的生成,抑制免疫反应,对大鼠坐骨神经crush损伤修复产生促进作用。
[ Objective] To study the effect of Simvastatin on the regeneration of sciatic nerve of rats and its mechanism of immunoregulation. [ Method ] Twenty eight female Sprague-Dawley rats were used and the rats experienced the sciatic nerve crush injury. Animals were randomized into： Simvastatin-treated crush injury animals, vehicle-treated crush injury animals and shamoperated animals. Simvastatin （20 mg/kg） was given once a day over a time period of 14 day sby oral garage via a pharyngeal tube. After surgery, the functional evaluation of nerve recovery, electrophysiologic assessment, histological assessment, serum IL- 6 and TNF-α assessment were performed. [ Result] The toe spread index of Simvastatin-treated crush injury animals was higher significantly than that of vehicle rats at d5 and d8 （ P 〈 0.05 ） postoperatively. CMAP was higher and NCV was faster （ P 〈 0. 05 ） ; The serum IL-6 and TNF-α of at 5d of post-operation was significant lower, especially IL-6. The pathological changes showed the numbers of myelinated axons of Simvastatin-treated crush injury animals were more than that of vehicle animals. [ Conclusion]The present study showed that Simvastatin may promote the regeneration of the sciatic nerve after crush injury in rats partly through lowering the levels of serum IL-6 and TNF-α.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2006年第24期1891-1893,1905,共4页
Orthopedic Journal of China
基金
大连市科技计划资助项目(No.2003B3NS222)
