摘要
目的:研究白黎芦醇对大鼠脑缺血/再灌注损伤后炎症的影响。方法:缺血前给予白黎芦醇10,20,40mg.kg-1,灌胃7 d,末次给药1 h后制备大鼠大脑中动脉阻断短暂局灶性缺血模型。脑缺血2 h再灌注24 h后,用TTC染色法、伊文思蓝法(EB)测定脑梗死范围及血脑屏障的损伤程度。分别用放免、酶免法测定大脑缺血区肿瘤坏死因子α(TNF-α)、髓过氧化物酶(MPO)的活性、血清白介素6,1β(IL-6,IL-1β)的含量。结果:白黎芦醇能缩小脑缺血/再灌注后脑梗死体积,减轻神经损伤症状,降低脑内TNF-α,MPO的活性及血清IL-6,IL-1β含量。结论:白黎芦醇可通过抑制脑缺血/再灌注过程中炎症介质释放,降低脑缺血/再灌注所致脑损伤。
Objective： To investigate the protective effects of resveratrol on inflammatory process induced by focal cerebral ischemia-reperfusion in rats. Method： Rats were pretreated with resvreratrol at the dose of 10, 20, 40 mg . kg^-1 for 7 days and then subjected to cerebral ischemia/reperfusion induced by a middle cerebral artery occlusion （MCAO）. The infarct volume and the neurological deficit were determined by the method of TTC （2, 3, 5-triphenylterazolium chloride） staining and Longa＇s score. The permeability of blood-brain barrier （BBB） was evaluated by measurement of the evans blue （EB） content in the brain with spectrophotometer. The content of interleukin-1β, interleukin-6 （IL-6, IL-1β） in serum and tumor necrosis factor-α （TNF-α）, myeloperoxidase （MPO） in brain were determined by radio-immunoassay and ELISA assay. Result： Resveratrol reduced infarct volume, ameliorated the neurological deficit and the permeability of BBB, the content of IL-6, IL-1β in serum and TNF-α, MPO activity in brain tissue also were significantly decreased. Conclusion： These results showed that resveratrol had protective effects on cerebral injury by inhibiting the releasing of the inflammatory mediators after ischemia/reperfusion injury.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第17期1792-1795,共4页
China Journal of Chinese Materia Medica
关键词
白黎芦醇
白介素1Β
白介素6
肿瘤坏死因
脑缺血
resveratrol
interleukin-1β
interleukin-6
tumor necrosis factor-α
brain ischemia
