摘要
目的探讨热适应对热应激细胞周期的影响。方法将人肝癌细胞系7 721细胞随机分为对照组、直接热暴露组、热适应组和热适应后热暴露组,分别给予不同的热处理,用流式细胞分析法检测细胞周期。结果热适应后43℃,暴露组G0/G1期细胞所占比例明显低于其他组(P<0.05),直接43℃暴露组G2/M期细胞所占比例明显低于热适用后暴露组和对照组(P<0.05),热适应后43℃暴露组S期细胞所占比例明显高于单纯热适应组和对照组(P<0.05)。结论热适应处理不仅可有效阻止热应激诱导的G0/G1细胞周期阻滞的发生,而且可增强热应激诱导的G2/M期和S期细胞周期阻滞的发生。
Objective To investigate the effect of heat adaptation on cell cycles of human hepatoma cells, Methods The 7721 cells were randomly divided into four groups, which were control group, heat adaptation group, heat exposure group, and heat adaptation ＋ heat exposure group. The cell cycles were determined by flow cytometry. Results The proportion of cells in G0/G1 phase in the group of heat acclimation ＋ heat stress was significantl less than those of other groups（P 〈 0.05）. The proportion of cells in G2/M phase in the group of directly exposure to 43 ℃ was obviously less that that in the group of heat acclimation ＋ heat stress（P 〈 0.05）. The rate of cells in S phase in the group of heat acclimation ＋ heat exposure was remarkably more than thus in the groups of the heat adatation only or control（P 〈 0.05）. Conclusion Heat adaptation could reduce the G0/G1 phase arrest, and improve the G2/M phase arrest and S phase arrest.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2006年第12期1433-1434,共2页
Chinese Journal of Public Health
关键词
热适应
热应激
细胞周期
细胞周期阻滞
heat adaptation
heat stress
cell cycle
cell cycle arrest
