摘要
目的通过对与免疫固定电泳、血清蛋白电泳、免疫球蛋白定量和热沉淀反应法这4种方法的比较,评价本周氏蛋白电泳在M蛋白检测中的价值。方法对178份尿标本同时采用热沉淀反应法和本周氏蛋白电泳检测本周氏蛋白;对25份多发性骨髓瘤患者的血清标本,同时进行本周氏蛋白电泳、血清蛋白电泳、免疫球蛋白定量和免疫固定电泳检测,比较其M蛋白的检出率和对M蛋白分型的作用;对29例出现M蛋白的患者的血和尿本周氏蛋白电泳结果进行了比较分析。结果本周氏蛋白电泳检测尿本周氏蛋白的敏感性远高于热沉淀反应法;免疫固定电泳和本周氏蛋白电泳检测血M蛋白的敏感性最高,血清蛋白电泳次之,透射比浊法免疫球蛋白定量再次之;同一患者血和尿标本的M蛋白出现情况并不一致。结论本周氏蛋白在M蛋白检测方面具有敏感性高,反映M蛋白成分全面的优点,最适合于M蛋白的筛查。血和尿标本中M蛋白出现并不一致,应同时检测。
Objective To evaluate the value of Bence - Jones protein electrophoresis in detecting monoclonal immunoglobulin by comparing with immunofixation electrophoresis, serum protein electrophoresis and quantitative assay of immunoglobulin. Methods Bence - Jones protein electrophoresis and heat test for urinary protein analysis were employed for detection of Bence - Jones protein in 178 urine samples; Benee - Jones protein electrophoresis, serum protein eleetrophoresis, immunofixation electrophoresis and quantitative assay of immunoglobulin were also used for detection and typing of proteins in 25 serum samples of multiple myeloma. Results Benee - Jones protein electrophoresis was more sensitive than that of heat test in detection of Benee - Jones protein in urine. Bence - Jones protein eleetrophoresis and immuonofixation electrophoresis were most sensitive in detection of monoclonal protein, next were serum protein electrophoresis and quantitative assay of immunoglobulin. Conclusion Bence - Jones protein electrophoresis is sensitive and can detect all monoclonal protein, thus it is suitable for screening of monoclonal immunoglobulin.
出处
《中国热带医学》
CAS
2006年第12期2180-2181,共2页
China Tropical Medicine
基金
中山市科学技术局科研资助立项(2005A090)
关键词
M蛋白
免疫固定电泳
多发性骨髓瘤
Monoclonal immunoglobulin
Immunofixation eleetrophoresis
Multiple myeloma
