摘要
目的：观察克罗米酚（ＣＣ）对子宫内膜不同类型细胞内雌激素受体（ＥＲ）和孕激素受体（ＰＲ）的影响。方法：采用抗ＥＲ、ＰＲ单克隆抗体的链霉菌抗生物素蛋白－过氧化酶连结法的免疫组化法，对１６例因不明原因（１１例）或男性因素（５例）不孕妇女的自然周期和用药周期的增生晚期和分泌晚期子宫内膜细胞内ＥＲ、ＰＲ，进行定位和半定量分析。结果：用药周期中，增生晚期和分泌晚期的内膜上皮细胞、间质细胞内ＥＲ和ＰＲ含量，均显著低于自然周期（Ｐ＜０．０１，Ｐ＜０．０５），且上皮细胞内受体含量较间质细胞明显低下（Ｐ＜０．０５），在增生晚期ＥＲ低下显著，在分泌晚期ＰＲ低下显著。结论：ＣＣ通过抑制ＥＲ和ＰＲ的合成，影响子宫内膜的生长。
Objective:Toassesstheefectsofclomiphenecitrate(CC)onestrogenreceptors(ER)andprogesteronereceptors(PR)expressionsinglandular,epithelialandstromalcelsofen-dometrium.Methods:Endometriumsamplesof16normalovulatorywomen(11withunexplainedand5maleinfertility)weretakeninthelateproliferativephase(12thday)andlatesecretoryphase(10daysafterovulation),bothinspontaneousandCC-inducedcycles.ERandPRinglandular,ep-ithelialandstromalcelsofendometriumwerelocalizedandtheirhistologicalscore(H-score)weremeasuredsemi-quantitativelybymonoclonalantibodiesimmunohistochemicalanalysis.Results:TheH-scoreofERandPRcontentsweresignificantlylowerinlateproliferativephaseandsecretoryphaseofCCcycleswhencomparedwiththatinspontaneouscycles(P<0.01,P<0.05).Thedecreaseofreceptorcontentsinglandularcelswassignificantlypronouncedthanthatinstromalcels(P<0.05).InlateproliferativephasethedecreaseofERwassignificantlyseverethanthatofPR.OnthecontraryinlatesecretoryphasethedeclineofPRwasmoresignificant.Conclusions:Basedonthesedata,wesuggestedthatCCinhibitedtheERandPRinductioninendometriumandafectedendome-trialdevelopment.ThesemaybeoneofthecausesoflowpregnancyrateinCC-inducedcycles.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
1996年第10期606-609,共4页
Chinese Journal of Obstetrics and Gynecology
关键词
克罗米酚
子宫内膜
雌激素
孕激素
受体
ClomipheneReceptors,estrogenReceptors,progesteroneEndometriumImmunohistochemistry
