摘要
目的 探讨慢性冬眠心肌(chronic hibernating myocardium,CHM)组织中胞外信号调节蛋白激酶(extracellular-signal regulated kinase,ERK1/2)的蛋白表达及其磷酸化形式(p-ERK1/2)的变化和意义。方法 10只小型中国家猪随机分为实验组(EXP,n=6)与对照组(CON,n=4)。实验组以右冠状动脉为靶血管,经右股动脉送入自制的缩窄器后继续喂养一个月制备成慢性冬眠心肌的模型。处死动物前三天采用硝酸甘油介入^(99)Tc^m-MIBI(^(99)锝~m-甲氧基异丙基丙腈)心肌灌注显像(SPECT)方法检测慢性冬眠心肌,获取对照组心肌组织(CON)、实验组中的正常心肌组织(NM)及慢性冬眠心肌组织(CHM)的样本进行光镜、电镜检查。采用免疫印迹(Western blotting)分析三组心肌组织中ERE1/2的蛋白表达及p-ERK1/2的变化。结果CHM中p-ERK1/2明显增高,与NM、CON中p-ERK1/2相比较有显著性差异(p<0.05,p<0.01)。结论 慢性冬眠心肌组织中ERK1/2被激活。
Objective To investigate the variation and significance of the member of mitogen-activated protein kinase （ MAP Kinase） superfamily-extracellular signal regulated kinase （ ERK1/2） and phosphorylated ERK1/2 （p-ERK1/2） in pig＇s chronic hibernating myocardium（CHM）. Methods 10 little domestic Chinese pigs were divided into experiment group（n-6,EXP） and control group（n= 4,CON） by random. The constrictors were sent into right coronary arteries of animals of experiment group and the animals were feed one month to establish an animal model of CHM. Dectecting CHM by nitroglycerion ^99TC^m-MIBI SPECT（ single-photo emission computed tomography）three days before the animals were killed. Verification the accuracy by observing the samples of CHM under microscope and electron microscope. Assessing the variation of ERK, p-ERK in nomal myocardium （NM）, CHM, and CON by Western blotting. RESULT p-ERK 1/2 were significantly increased in CHM compared with NM and CON （ p 〈 0.05, p 〈 0.01 ） . CONCLUSION ERK were activated in tissues of CHM.
出处
《中国分子心脏病学杂志》
CAS
2003年第1期28-31,共4页
Molecular Cardiology of China
基金
江苏省应用基础研究项目BJ2000059
关键词
慢性冬眠心肌
ERK1/2
超微结构
Chronic hibernating myocardium extracellular signal regulated kinase（ERK1/2）Ultrastructure
