摘要
目的:建立测定白花丹参中丹参酮Ⅱ_A 和隐丹参酮含量的方法。方法:高效液相色谱法,采用 Diamonsil C_(18)色谱柱(150mm×4.6mm,5μm),流动相为甲醇-水(85:15),流速0.8mL·min^(-1),柱温35℃,检测波长为269nm。结果:丹参酮Ⅱ_A 在0.07～0.75μg范围内线性关系良好(r=0.9998),平均同收率为98.5%,RSD 为2.0%(n=5);隐丹参酮在0.04～0.43μg范围内线性关系良好(r=0.9994),平均回收率为102.1%,RSD 为1.7%(n=5)。结论:本法简便、可靠、准确。为白花丹参的质量评价提供了依据。
Objective ： To develop a new method for the determination of tanshinone ⅡA and cryptotanshinone in root of Salvia miltiorrhiza Bge. f. alba. Methods：RP - HPLC was conducted on a Diamonsil C18 colomn （ 150 mm× 4. 6 mm,5 μm） , with mobile phase methanol - water （ 85： 15 ） , flow rate 0.8 mL · min ^-1 , detecting at 269 nm and column temperature at 35 ℃. Results ： The linear range was 0. 07 - 0. 75 μg （ r = 0. 9998 ）, the average recovery was 98.5 %, RSD = 2.0% （ n = 5 ） for tanshinone Ⅱ A and the linear range was 0. 04 - 0.43 μg （ r = 0. 9994）, the average recovery was 102. 1% ,RSD = 1. 7% （n = 5 ）for cryptotanshinone. Conclusion： The method is accurate, simple and effective. It can be used as a quality evaluation in Salvia miltiorrhiza Bge. f. alba.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2006年第10期1401-1403,共3页
Chinese Journal of Pharmaceutical Analysis
基金
山东省"中药材新品种规范化种植与开发"项目(SDGP2004-54)
关键词
RP—HPLC
白花丹参
丹参酬ⅡA
隐丹参酮
RP - HPLC
Salvia miltiorrhiza Bge. f. alba
tanshinone ⅡA
cryptotanshinone
