摘要
应用血清学的酶联免疫吸附法(ELISA),对采自四川省内4个葡萄主产区内的34个葡萄品种,共计199份枝条样本进行了葡萄病毒病的检测和分析。在被测试的26个鲜食葡萄品种中,18个品种被1种或多种危险性的目标病毒所感染,品种感染率为69.2%;而在8个酿酒葡萄品种中,仅1个品种感染了危险性病毒,感染率为12.5%。在5个受测目标病毒和类型中,GLRaV-3的发生和危害最为普遍,其单个采样点的样本感染率为25.5%￣56.3%,其次为GVA0￣25.5%,GVB0￣12.5%和GLRaV-20￣4.5%,GFLV在本次试验中未被检测到。在每个取样点,感病品种一般所有单株都被同一种病毒感染,因此葡萄繁殖材料的引入是危险性病毒进入新产区的主要途径;而在生产实践中,高接换种方式会成倍增加葡萄病毒传播侵染的风险性。
199 stem samples of 34 grape cuhivars collected randomly from d major producing areas in Siehuan province were tested and identified for their sanitary state by Das or Pas-ELISA. Among 26 table cuhivars, 18 were found infected by at least 1 dangerous target virus, so the infection rate was 69.2%. In 8 wine cuhivars, only 1 was found infected by dangerous virus, so the infection rate was 12.5%. Among 5 target viruses or types, GLRaV-3 was found most widely spread in the 4 producing areas, its sample infection rate of single site ranged from 25.5%-56.3%, followed by GVA from 0-25.5%, GVB from 0-12.5% and GLRaV-2 from 0-4.5%, but GFLV had not been found. In each site, almost all samples of every infected cultivar were found to be infected by the same virus, which suggested that introduction of grapevine propagation materials into an new producing area should be responsible for the invasion of new virus disease. In local cultivation practices, top grafting was often applied to replace old grape cuhivar, this had made the virus disease transmission from one cuhivar to another even worse.
出处
《果树学报》
CAS
CSCD
北大核心
2006年第6期846-849,共4页
Journal of Fruit Science
基金
中意政府间合作项目"四川果苗繁育中心"(74-16.07.1999)
四川省财政厅高新技术专项(140102)。
关键词
葡萄品种资源
病毒病鉴定
ELISA
健康状况评估
Grape cultivar
Identification of virus diseases
ELISA
Evaluation of sanitary state
