摘要
6-BA、TDZ与IBA不同浓度组合对美人指葡萄叶片、叶柄和茎段不定芽离体器官发生途径诱导再生植株有不同的影响。以茎段为外植体在MS+6-BA1.0mg/L+IBA0.01mg/L培养基上分化效果最好,再生率75.00%;叶片、叶柄分别在MS+6-BA3.0mg/L+IBA0.10mg/L和MS+6-BA2.0mg/L+IBA0.05mg/L培养基上分化效果较好。TDZ和IBA研究的几种组合都能诱导不定芽再生,但再生率不高。
Plant regeneration were induced from leaf, petiole and stem explants of grape cuhivar Manicure finger （Vitis vinifera L.） with MS medium supplemented different combination of 6-BA, TDZ and IBA through organogenesis. The results indicated that the highest regenerating rate of adventitious buds were obtained when stem explants were incubated on MS media with 6-BA 1.0 mg/L＋IBA 0.01 mg/L, it reached 75.00%. The optimum media for inducing adventitious buds from leaf and petiole explants were MS＋6-BA 3.0 mg/L＋IBA 0.1 mg/L and MS＋6-BA 2.0 mg/L＋IBA 0.05 mg/L. The initiation media containing various concentrations of TDZ and IBA could induce adventitious buds from leaf, petiole and stem explants, but the rates were not so high.
出处
《果树学报》
CAS
CSCD
北大核心
2005年第5期551-553,F0003,共4页
Journal of Fruit Science
基金
江苏省科技厅
省农业资源开发局科技推广资助项目。
关键词
美人指葡萄
不定芽
植株再生
Manicure Finger Grape
Adventitious buds
Plant regeneration
