摘要
血管内皮细胞损伤是创伤、感染等休克过程中最早出现和最常见的形态学变化,其机制较复杂,参与因素也较多。低分子纤维蛋白(原)降解物(Lmw FDP)可能是其损伤中有重要因素之一。本研究采用HPLC法检测了LmwFDP成分之一——FPA、FPB于烧伤后的变化规律,同时利用体外培养内皮细胞的方法,观察了烧伤血清和FPA、FPB对内皮细胞的损伤作用。本实验主要结果如下: (1)将烧伤血清和灭活补体的烧伤血清分别与内皮细胞孵育,内皮细胞变性,坏死,培养液中LDH和6-酮-PGF_(1α)含量升高。说明烧伤血清对内皮细胞有毒性作用,且此毒性不仅仅是通过补体介导的。 (2)本实验观察18例烧伤病人(TBSA为46.66±21.0％)发现:烧伤后2天,血清FPA、FPB升高,5～7天达高峰,15天开始恢复,25天接近对照水平。伤后FPA、FPB的变化与病情进展有密切关系。因而检测烧伤后血清FPA、FPB的变化,有助于了解病情。 (3)体外实验表明:FPA、FPB可损伤血管内皮细胞,其损伤作用特点如下:①不可逆性。②剂量依赖性。③特异性。上述结果表明:FPA、FPB在内皮细胞损伤中可能具有重要作用。
Endothelial injury appears very early after traumatic and toxic shock. The mechanism and etiology are-complex.The low-molecular weight fibrinogen degradation-products (LmwFDP)may be one of the important factors.Dynamic changes of fibrinopeptide A and B (FPA, FPB) in sera of burn patients (BPS) are determined with HPLC method. On this basis detrimental effects of BPS and FPA, FPB on endothelial cells are observed in vitro. The main results are as follows: 1) after being incubated with BPS or the sera with complements being inactivated by heatine at 56 degrees C for 30 min, the endothelial cells (EC) all become denatured and necrotized, and the levels of 6-keto-PGF1α and LDH in the media are higher than those measured before incubation. This indicates that the presence of the factors other than complements, in the BPS injurious to the cultured EC; 2) FPA and FPB in 18 BPS become increasing at 2 postburn day (PBD) , reach their peaks at 5-7 PBD, then decline after 15 PBD,and return to the control levels at 25 PBD.The fluctuation of FPA,FPB levels in BPS are parallel to the progress of burn illness. The measureements of FPA, FPB levels in BPS may be useful in evaluating patients condition. 3) FPA and FPB are detrimental to the cultured EC in vitro. Characteristics of their injurious effects are:inreversible,dose dependent,and specific. The FPA and FPB may play an important role in endothelial injury.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1990年第4期275-282,共8页
Journal of Third Military Medical University
关键词
烧伤
纤维蛋白肽
血管内皮细胞
burns/PP
blood/AN
fibrinopeptides A
fibfinopeptides B
endothelium
