摘要
我们以反相高效液相色谱法(RP-HPLC)和放射免疫测定法(RIA)研究了粉防己碱(Tet)对体外用钙离子载体A_(23187)诱导兔多形核白细胞(PMN)合成白三烯B_4(LTB_4)和血栓素A_2(TXA_2)的影响,并以地塞米松(Dex)和氟奋乃静(FPZ)作对照。结果表明,Tet与PMN温育15min即可抑制PMN合成LTB_4和TXA_2,其效应呈剂量依赖性。加入外源性花生四烯酸(AA)时,Tet的上述抑制作用消失。FPZ与Tet有相似的作用,Dex则与Tet不同,它需与PMN温育60min才出现抑制作用。
The effects of tetrandrine (Tet) on the production of leukotrine B4(LTB4) and thromboxaneB2(TXB2)of calcium ionophore A23187-stimulated rabbit polymorphonuclear leukocytes (PMN)are measured with the reversed phase high performance liquid chromatography and radioimmunoassay respectively. Dexa-methasone (Dex) and fluphenazine (EPZ)are used as the control. The production of LTB4 and TXB2 by PMN is significantly depressed by Tet in a dose-dependent mannet when incubated for 15 min. The IC50 for inhibition of LTB4 and TXB2 production is 8.3 and 9.6 μmol/L respectively. The characteristics of the effect of Tet are the same as those of FPZ.The effect of Dex occurs when incubated with PMN for 60 min.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1990年第4期291-29,共1页
Journal of Third Military Medical University
关键词
粉防己碱
花生四烯酸
多形核白细胞
tetrandrine
arachidonic acid/ME
leukotrine B
thromboxaneB2
neutrophils
A-23187
