摘要
目的：观察Ｉｎｔｒａｌｉｐｉｄ对内毒素所致的多形核白细胞氧自由基产生的影响。方法：取２２例健康志愿者静脉血，采用Ｂｏｙｕｍ法提取多形核白细胞（ＰＭＮ），并在体外培养。采用不同剂量的脂多糖（ＬＰＳ）刺激ＰＭＮ，并和不同剂量Ｉｎｔｒａｌｉｐｉｄ共同作用。采用ＮＢＴ还原试验测定氧自由基的产生量。结果：ＬＰＳ刺激各组的ＮＢＴ值明显高于非刺激组，而且ＮＢＴ值与ＬＰＳ浓度明显相关。Ｉｎ－ｔｒａｌｉｐｉｄ可明显降低ＮＢＴ值，且ＮＢＴ值与Ｉｎｔｒａｌｉｐｉｄ浓度呈负的线性相关。结论：不论有无内毒素刺激，Ｉｎｔｒａｌｉｐｉｄ在体外均可抑制吞噬细胞产生氧自由基。
Objectives: To study the effect of Intralipid on the release of oxygen radicals from phagocytes in vitro. Methods: Human polymorphonuclear neutrophils(PMN) were purified and incubated either with purified lipopolysaccharides(LPS) or with Intralipid.The release of oxygen radicals was measured by the nitroblue tetrazolium(NBT) test. Results: The incubation of PMN with LPS increased the release of oxygen radicals.However,when the PMN were incubated with LPS together with Intralipid,the release of oxygen radicals was significantly decreased. Conclusions:Intralipid might inhibit the effect of LPS on the PMN in an infection with Gramnegtive bacteria.
出处
《肠外与肠内营养》
CAS
1999年第3期138-140,共3页
Parenteral & Enteral Nutrition
