摘要
TNF和LPS对机体都具有毒性,尤其对荷瘤宿主毒性更强。在S_(180)肉瘤接种后第10 d动物静脉输注5μg TNF或25 μg LPS并未致使荷瘤小鼠死亡,但5μg LPS和0.5 μg TNF联合运用导致了80％荷瘤小鼠死亡。这种联合毒性对LPS剂量有较严格依赖性,当LPS减少到1 μg后,联合致死率锐减到14％。而TNF减少到0.1 μg后,动物死亡率仍高达50％。提示TNF在协同致死毒性中起到致敏机体,增加宿主对LPS毒性敏感性的作用。消炎痛处理可以部分阻断这种联合致死作用,说明这种联合致死毒性是部分通过前列腺素介导的。
Both TNF and LPS are toxic to host, especially to tumor - bearing host Intravenous administration at a dose of 5μg of TNF or 25μg of LPS could not result in death of mice transplanted with sarcoma 180 (S180) 10 d later, but 80% of S180-bearing mice died after iv injection of 5μg of LPS combined with 0.5μg of TNF. In contrast to TNF, combined toxicity was seriously LPS-dose dependent Combined mortality dropped from 80% to 14% when LPS dose reduced to 1μg, however, 50% of mice died of combined toxicity when only TNF dose dropped to 0.1μg. It suggested that TNF sensitized host response to toxic effect of LPS. Indomethacin could partially block the combined toxicity, demonstrating that combined toxicity of LPS and TNF was partially mediated by prostaglan-dins.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1990年第4期328-330,共3页
Academic Journal of Second Military Medical University
关键词
肿瘤坏死因子
细菌脂多糖
致死毒性
tumor necrosis factor
lipopolysaccharide
combined lethal toxicity
mice
