摘要
采用横切薄片培养方法建立了香蕉基因转化的适宜受体系统.结果表明:在30℃、黑暗培养15 d,而后转入24℃、光照培养5 d的条件下,香蕉横切薄片的出芽率较高,植株生长较健壮,适宜用作转基因的受体;羧苄青霉素对香蕉薄片的生长没有明显的抑制作用,可以作为转化的抑菌剂;香蕉对潮霉素比较敏感,能够显著抑制香蕉横切薄片的出芽率,确定用40mg.L-1作为潮霉素的筛选含量.
An appropriate transgenie aeceptor system for banana （Musa spp. ） was developed in this experiment. The high efficiency of shoot differentiation was obtained from the thin-cross-section cultured at 24℃ in light for 5 days after being cultured at 30 ℃ in darkness for 15 days. The regenerated plantlets grew well and the thin-cross-sections of the plantlets could be used as transgenic acceptors for Agrobacterium-mediated transformation. Carbenicillin could not inhibit growth and differentiation of the thin-cross-sec- tions of banana and could be used to kill Agrobacterium during the selection stage. Banana was sensitive to hygromycin B, and hy- gromycin B could inhibit growth and differentiation of the thin-cross-sections of banana, which could be used as a selection agent for banana transformation. The results showed that 40 mg · L^-1 hygromycin was the lowest concentration for the selection.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2006年第5期509-514,共6页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
福建省科技厅重大科技专项项目(2004NZ02-2)
关键词
香蕉
转基因受体系统
薄片培养
banana
transgcnic acccptor system
thin-cross-section culture
