摘要
目的检测用两种不同方法提取的昆虫抗菌肽对作用细胞的影响。方法对家蝇幼虫采用针刺感染大肠埃希菌诱导产生抗菌肽,经研磨、层析提取其抗菌肽,并通过流式细胞仪测定用生理盐水或磷酸缓冲液这两种方法提取的抗菌肽对癌细胞和正常细胞的影响。结果两种方法处理的家蝇幼虫抗菌肽对人髓样白血病细胞(K562)均有杀伤作用;而对正常人淋巴细胞,生理盐水处理的抗菌肽比缓冲液伤害作用小。结论缓冲液可能对细胞造成高渗作用促进细胞死亡;而生理盐水无此作用,对细胞不造成伤害。
Objective To compare the two mothods of isolating the antimicobial peptides from Musca domestica larvae. Motbods Musca domestica larvae were infected with Escherichia coli by a sting, then the antimicrobial peptides were extracted from the insect. After that, the antimicrobial peptides were isolated by grinding, sephadex G-50 gel filtration. The last, the anticancer activity of the peptides worked upon K562 cell and lymph cell were detected by Flow cytometer（FCM）. Results The two kinds of peptides from Musca domestica isolated by the different methods could kill K562 cell to some extent, but they are different in working upon normal cell. Physiological saline influence cell less than the buffer. Conclusion Buffer could make a high osmotic pressure and impel cell to death; physiological saline don＇t do as the buffer.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
北大核心
2006年第5期385-387,共3页
Chinese Journal of Vector Biology and Control
基金
山西省科技开发基金(晋教计财[2003]46)
关键词
提取方法
伤害
细胞
Extracting method
Influence
Cell
