摘要
目的研究棕榈酸对大鼠主动脉内皮细胞凋亡的影响。方法 0.5%BSA或0.5%BSA及不同浓度的棕榈酸(0.1、0.2和0.3 m m)作用于大鼠主动脉内皮细胞24 h,M TT法检测细胞活性,流式细胞仪AnnexinⅤ/PI法检测细胞凋亡,分光光度计检测C aspase-3活性。结果 M TT检测结果提示棕榈酸组较对照组细胞活性下降;AnnexinⅤ/PI法检测结果提示棕榈酸组较对照组细胞凋亡增加(P<0.05或P<0.01)。棕榈酸组较对照组C aspase-3活性增加(P<0.05或P<0.01)。结论棕榈酸能够诱导内皮细胞凋亡,说明高水平的游离脂肪酸对内皮细胞有直接的伤害作用。
【Objective】We investigated the effect of ghrelin on palmitate-induced apoptosis in rat aortic endothelial cells.【Methods】Rat aortic endothelial cells were incubated in 0.5% BSA or 0.5% BSA complexed to palmitate（0.1 mm,0.2 mm,0.3 mm）.Cell viability was assessed by MTT assay.Apoptosis was detected using an annexin V/PI double staining assay.Spectrofluorometer assay caspase-3 activity.【Results】Palmitate decreased cell viability compared with control group as assessed by MTT asssy.Palmitate increased cell apoptosis compared with BSA group as assessed by an annexin V/PI double staining assay（P 0.05 or P 0.01）.Palmitate increased caspase-3 activity compared with BSA group（P 0.05 or P 0.01）.【Conclusions】These findings provide the evidence that palmitate could induce apoptosis in rat aortic endothelial cells.High level of free fatty acid could injury endothelial cells directly.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2012年第8期19-22,共4页
China Journal of Modern Medicine
关键词
棕榈酸
凋亡
内皮细胞
palmitate
apoptosis
endothelial cells
