摘要
目的表达HIV-1型gag基因蛋白。方法将编码HIV-1型gag的部分序列,克隆到表达载体pET-28 a后,进行IPTG诱导在E.coli BL21(DE3)表达,表达产物分别经SDS-PAGE和W estern-B lot进行检测。结果表达产物为分子量约50 kD的融合蛋白,并可与HIV-1型病人阳性血清发生特异性反应。结论gag基因在大肠杆菌中得到表达,且表达产物具有良好的抗原性。
Objective To express HIV-1 gag protein in E. Coll. Methods The truncated gag gene was inserted into the expressing vector Pet-28a and E. coli B1.21 （ DE3 ） was then transformed and IPTG was induced. The expressing product by SDS-PAGE and Western-Blot was detected. Results The recombinant plasmid was expressed with about 50 kD fusion protein which could react specifically with the HIV positive serum. Couclusion The truncated HIV-1 gag gene can be expressed in E. coli and the recombinant protein has positive antigenicity.
出处
《临床肺科杂志》
2006年第6期769-770,共2页
Journal of Clinical Pulmonary Medicine
