摘要
用聚合酶链反应(PCR)技术检测19份急性弛缓性麻痹患者(AFP)的粪便中脊髓灰质炎(脊灰)病毒,其中11份阳性,与中和试验鉴定及单克隆抗体鉴定结果符合率为90.90%～100%。该法不仅简便、敏感、特异分型,而且可作型内鉴别,为环境样品中脊灰病毒污染提供了检测手段。
Poliovirus-RNA was detected in 11 clinical fecal specimens with AFP out of 19 samples. These comparative results between NT and Mcah identifications showed a coincidence rate of 90.90%-100%. Our experiment suggested that PCR a simple ,sensitive,identification type and could be used for intratypic differentiation of poliovirus strains. It can also be applied for the detection of poliovirus contamination in the environment,
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
1996年第3期166-168,共3页
Chinese Journal of Epidemiology
