摘要
为探索乙型肝炎病毒（ＨＢＶ）前Ｃ区突变是否与肝损程度及肝癌发生有关，对１３９例ＨＢ－ｓＡｇ、ＨＢＶＤＮＡ和抗－ＨＢｅ阳性，ＨＢｅＡｇ阴性的慢性ＨＢＶ感染者和肝癌患者的血清标本，采用３’碱基特异性聚合酶链反应（３’－ＢＳ－ＰＣＲ）进行了第１８９６位核苷酸突变的检测分析。结果１３９例中４１例（２９．５％）检出了ＨＢＶ突变株，无症状ＨＢＶ携带者、慢性迁延性肝炎（慢迁肝）、慢性活动性肝炎（慢活肝）和重型肝炎（重肝）组的检出率分别为８．７％、１３．１％、１９．６％和３９．１％。慢活肝和重肝组明显高于携带者（Ｐ＜０．０５），肝癌患者的检出率为７７．３％，显著高于其他各组，提示ＨＢＶ前Ｃ区１８９６位核苷酸突变与严重肝损害和肝癌的发生可能有一定关系。
The aim of this study is to explore the relationship between precore mutation of HBV and severity of liver damage as well as occurrence of HCC,The G to A mutation at nucleotide l896 in the pre C region of HBV DNA has been detected in l39 serum samples of patients with persistent HBV infection by a simple method3’BS-PCR,All sera were HBsAg、HBV DNA,and anti-HBe positive-The mutation of pre C was detected in 4l out of l39 cases(29.5%).The prevalence of pre C of ASC,CPH,CAH,SH,and HCC wsa 8.7%,13.3%,19.6%,39.1%and77.3%respectively.A significant difference was found between CAH, SH and ASC(P<0.05),and between HCC and other groups(P<0.005).These results suggest that the pre C mutation at nucleotide l896 of HBV may contribute to the serious liver damage and HCC
出处
《中华传染病杂志》
CAS
CSCD
北大核心
1996年第1期11-14,共4页
Chinese Journal of Infectious Diseases
