摘要
目的:建立安中片的质量控制方法。方法:采用 TLC 方法对安中片中的延胡索、甘草进行定性鉴别,采用 HPLC 法,All-tima C_(18)色谱柱(250mm×4.6mm,5μm),以甲醇-1%醋酸(45:55)为流动相,检测波长285nm,柱温40℃,流速1mL·min^(-1),进样量10μL,对安中片中的肉桂酸进行了含量测定。结果:延胡索、甘草的薄层色谱鉴别专属性强,肉桂酸进样量在0.004～0.104μg范围内与峰面积呈良好的线性关系(r=0.9999,n=8)。肉桂酸的平均回收率为98.6%,RSD=1.6%。结论:本方法可准确地进行定性、定量,可用于控制安中片的质量。
Objective： To establish the quality control standard of Anzhong tablets. Method： Rhizoma Corydalis, Radix et Rhizoma Glycyrrhizae were identified by TLC. The content of cinnamic acid in Anzhong tablets was determined by HPLC. Alltima C18 column （250 mm × 4. 6 mm, 5 μm） was used. The mobile phase was methanol - 1% acetic acid（45：55 ） ,the detection wavelength was at 285 nm ,column temperature at 40 ℃ ,the flow rate was 1 mL · min^-1 ,and the injection volume was 10 μL. Result：The calibration curve was linear in the range of 0. 004 - 0. 104 μg for cinnamic acid （r = 0. 9999, n = 8 ）. The mean recovery of cinnamic acid was 98.6%, RSD = 1.6%. Conclusion：This method is simple, reliable, accurate, and used to control the quality of Anzhong tablets.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2006年第8期1065-1067,共3页
Chinese Journal of Pharmaceutical Analysis
