摘要
用聚丙烯酰胺均匀胶和等电聚焦电泳技术，分析了甜椒种子及种苗的过氧化物同功酶谱和种子水溶蛋白图谱。在过氧化物酶谱带的两个位点ＰＲＸ－１和ＰＲＸ－２上，自交系亲本与Ｆ１杂交种有明显的差异。水溶蛋白的一个位点ＰＲＯＴ亲本与Ｆ１有明显差异。供试甜椒组合的过氧化物酶和蛋白的图谱，分别具有这些位点。利用这些鉴别图谱，对甜椒商品种子样品进行的杂交种纯度检验，纯度范围在８％～９９％，与田间检测结果一致。
Peroxidase isozyme phenotypes and water soluble protein were determined in parental inbreds and corresponding F 1 hybrid seed lots of two commercial sweetpepper varieties to determine seed purity .The inbred lines differed with their F 1 hybids at two peroxidase loci (PRX-1, PRX-2) and one water soluble protein locus(PROT).Differences between inbred parents were found at least for one of the three loci.Models were developed to estimate seed genetic purity. The genetic purity was estimated range from 8% to 99%.These estimates conformed favorably with those obtained from field testing
出处
《华北农学报》
CSCD
北大核心
1996年第4期119-122,共4页
Acta Agriculturae Boreali-Sinica
关键词
甜椒
同功酶
水溶蛋白
纯度鉴定
杂交种
Sweet pepper
Isozyme
Water soluble protein
F 1 hybrid purity identification
