摘要
选择三种同源四倍体水稻为实验材料,研究了氮离子注入对其花药诱导愈伤分化成苗组织培养体系影响.结果显示,低剂量范围(1.0×1015~2.0×1015N+/cm2)的N+注入能明显提高愈伤诱导率.其可能原因为:低剂量N+注入可使花药组织的膜透性增强,新陈代谢加快,从而使诱导率增加.另外,无论注入剂量大小,N+注入都能明显改善愈伤生长状态,抑制其褐化衰老的进程,还能提高绿苗分化率.对此,从生化角度进行了一定的理论分析.
The effect of N^+implantation on autotetraploid rice anther tissue culture is studied with 3 materials.As a result,N^+implantation of low dosage(1.0×10^15-2.0×10^15 N^+/cm^2)can heighten callus-induced rate.The reason is that the effect of low dosage N^+implantation can stimulate membrane permeability and metabolism of anther tissue culture.Callus quality is distinctly improved,and browned process and caducity of callus are slowed down,and the callus productivity and green seedling differentiation rate are improved after N^+implantation.This phenomenon is analized from the viewpoint of biochemistry.
作者
李玉峰
梁运章
黄群策
LI Yu-feng;LIANG Yun-zhang;HUANG Qun-ce(Inner Mongolia Key Laboratory of Ion Beam Bioengineering,Hohhot 010021,China;College of Sciences and Technology,Inner Mongolia University,Hohhot 010021,China;Key Laboratory of Ion Beam Bioengineering,Zhengzhou:University,Zhengzhou 450052,China)
出处
《内蒙古大学学报(自然科学版)》
CAS
CSCD
北大核心
2006年第5期531-534,共4页
Journal of Inner Mongolia University:Natural Science Edition
基金
国家自然科学基金(10065001)
