摘要
目的：研究氟罗沙星在胶束体系中的荧光特性及应用．方法：用日立F-3010型荧光分光光度计测定，测定波长λex=290nm，λem=450nm，测定时最佳pH值范围为4．5～5．5．在表面活性剂十二烷基硫酸钠（SDS）存在下，由于胶束的生成，使氟罗沙星（FLRX）自身荧光大大增强，据此建立了在SDS胶束体系中测定氟罗沙星的新方法．结果：氟罗沙星浓度在0～0．9μg／mL范围内与荧光强度呈线性关系，相关系数0．9991，检测限为0．01μg·mL^-1．加标回收率为97．1％～102．5％，相对标准偏差为1．5％～3．6％．结论：该方法简便，快速，可直接用于药物制剂中氟罗沙星含量的测定．
Objective ： The fluorescence characteristics and its application of fleroxacin in micellar system have been studied. Methods： Hitachi F-3010 model spectrofluorimeter. The optimum pH range was 4. 5-5.5. The experiments indicated that sodium dodecyl sulfate micellar systems could greatly enhance the fluorescence intensity of fleroxacin. Thus,a new method has been developed for the determination of fleroxacin. Results：The fluorescence intensity was linearly related to the fleroxacin concentration in the range of 0- 0. 9 μg/mL with correlation coefficient 0. 9991. The determination limit was 0. 01 μg · mL^-1. The recovery was within the range of 97. 1%- 102.5% and the RSD was 1.5%-3. 6%. Conclusion： The method is simple and accurate,and can be used for the determination of fleroxacin in preparations.
出处
《内蒙古大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第5期513-515,共3页
Journal of Inner Mongolia University：Natural Science Edition
基金
内蒙古教育厅项目(NJ05040)
内蒙古教育厅项目(NJ05096)
关键词
氟罗沙星
胶束增敏
荧光光度法
药物制剂
fleroxacin
micellar enhanced sensitivity
fluorescence spectroscopy
pharmaceutical preparation
