摘要
【目的】研究不同冷缺血损伤条件下,肿瘤坏死因子(TNF-α)和白介素-6(IL-6)等细胞因子在大鼠移植肝内的变化规律与肝脏再生的关系。【方法】建立大鼠原位肝移植模型。实验分为:冷缺血1h组、冷缺血16h组、假手术对照组。观察各组的生存率,并在术后0、1、2、4、16、24、48、72h收集标本,检测TNF-α、IL-6等细胞因子的表达。免疫组化检测肝细胞摄取溴脱氧尿核苷情况。对冷缺血1h组和冷缺血16h组在移植后48h溴脱氧尿核苷染色阳性的肝细胞计数进行分析。【结果】冷缺血损伤1h、16h肝移植组和假手术组存活率均为100%(>14d)。与冷缺血1h相比,冷缺血16h组大鼠移植肝内TNF-α、IL-6等细胞因子表达明显增加,持续的时间也延长到移植后24h以上。移植术后48h溴脱氧尿核苷染色阳性的肝细胞计数也明显增多(P<0.05)。【结论】重度冷缺血损伤启动大鼠肝移植后的肝脏再生,修复组织损伤。与大鼠肝切除后诱导肝再生的机制相似,TNF-α、IL-6等细胞因子在移植后肝脏再生的早期启动过程中也非常重要。
[Objective] To explore the effect of different cold ischemia times on the patterns of intragraft cytokines of tumor necrosis factor-α（TNF-α） and interleukin-6（IL-6） and their correlation with liver regeneration after rat liver transplantation（LT）. [Method] Model of rat LT was established. The rats were divided into 3 groups： group of 1 h cold ischemia, group of 16 h cold ischemia and control group （sham-operated）. Survival rate of each group was recorded. The presence, and upregulation pattern of TNF-α and IL-6 were determined in liver grafts with minimaf（1 h） and prolonged（16 h） cold ischemia times following transplantation. Specimen were collected at predetermined intervals from 0, 1, 2, 4, 16, 24,48,and 72 h post reperfusion. Progression of DNA synthesis of hepatecyte was confirmed by bromodeoxyuridine （BrdU） uptake. Positively BrdU-stained neclei were quantitated and analyzed between the first two groups at 48 h post reperfusion. [Results] LT was successfully performed in experimental groups. Survival in all groups was 100% （〉14 d）. With 16 h cold ischemia, TNF-α and IL-6 expression were markedly increased and more prolonged up to 24 h posttransplantation. Positively BrdU-stained neclei were quantitated and analyzed between experimental groups. Number of positively stained neclei in 16 h cold ischemia group were more than those in 1 h cold ischemia group at 48 h after transplantation （P〈 0.05）. [ Conclusion ] Severe cold ischemia initiated liver regeneration and graft recovery after rat LT. Similar to that in the rat partial hepatectomy model, TNF-α and IL-6 are important factors in early initiating liver graft regeneration in the recovery of cold ischemia injury.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2006年第5期537-540,共4页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广东省卫生厅基金资助项目(A2001145)
