摘要
目的探讨基质金属蛋白酶(MMP-2)在糖尿病视网膜病变(DR)发生发展中的作用及其机制.方法用链脲佐菌素(STZ)诱导苍鼠糖尿病模型,提取视网膜中总RNA,半定量RT-PCR观察视网膜中MMP-2mRNA表达情况,HE染色观察光镜下视网膜的形态特征及变化,血清学检测苍鼠血清甘油三酯(TG),总胆固醇(TC)和电化学发光法检测胰岛素水平等变化.结果糖尿病苍鼠不仅表现为高血糖,还表现为高TG血症.视网膜中MMP-2mRNA表达呈升高趋势,与正常对照组相比于造模后16wk末时即差异具有统计学意义(P<0.05).结论此为研究基质金属蛋白酶参与DR的发病机制提供了依据.
AIM： To explore the role of matrix metalloproteinase-2 （MMP-2） in the development of diabetic retinopathy （DR）. METHODS： Hamsters were used to establish diabetic models by Streptozotocin （40 mg/kg）. Total RNA of retina was collected. The expression of MMP-2 mRNA in retina were observed by using semi-quantitative RT-PCR. Morphologic characteristics and changes of retina was observed by HE staining. The changes of TG, TC were tested serologically and insulin was tested by electrochemiluminescence. RESULTS： Diabetic hamster displayed not only hyperglycaemia, but also hyperlipemia. The expression of MMP-2 mRNA in retina showed a increasing tendency. CONCLUSION： MMP-2 may contribute to the pathogenesis of DR.
出处
《第四军医大学学报》
北大核心
2006年第17期1570-1572,共3页
Journal of the Fourth Military Medical University
