摘要
目的:探讨将细胞块技术应用于宫颈细胞学诊断并检测宫颈脱落细胞p16INK4A表达,对评价宫颈病变的意义。方法:采用链霉素抗生物-过氧化物酶(SP)法,检测79例细胞块上的宫颈脱落细胞及相应的宫颈活检组织中p16INK4A表达。结果:在宫颈脱落细胞中,p16INK4A在正常、未明确意义的不典型鳞状细胞(ASCUS)、低度鳞状上皮内病变(LSIL)、高度鳞状上皮内病变(HSIL)、鳞状细胞癌(SCC)阳性表达率分别为0%、18.18%、46.43%、89.66%、100%。在相应活检组织中,p16INK4A在宫颈炎、CINⅠ、CINⅡ、CINⅢ、SCC中阳性表达率分别为0%、61.90%、87.50%、100%、100%。p16INK4A在宫颈脱落细胞和组织中表达的符合率为87.34%,宫颈脱落细胞p16INK4A免疫组化染色使检出宫颈高度病变的敏感性提高了21.95%。结论:细胞块技术可用于宫颈细胞学诊断,检测宫颈脱落细胞p16INK4A表达可以提高宫颈高度病变的检出率,减少漏诊率。
Objective： To investigate the application of cell block preparations in cervical cytology and to assess the significance of detecting p16^INK4A expression in cervical deciduous cells on cell block to identify cervix lesion. Methods： Cell block be prepared from residual material of 79 Thinprep Preservcyt solutions streptavidin/peroxidase （SP） method was used to examine expression of p16^INK4A in cell block and corresponding biopsies at the same time. Results： Positive rate of immunostaining for p16^INK4A on cervical deciduous cell in normality, ASCUS, LSlL, HSIL and SCC were 0%, 18.18% ,46.43% ,89.66%, and 100%, respectively. Positive rate of Immunostaining for p16^INK4A in corresponding biopsies in cervicitis, CIN Ⅰ , CIN Ⅱ, CIN Ⅲ, and SCC were 0%, 61.90%, 87.50%, 100%, and 100%, respectively. The coincidence rate of p16^INK4A expressed in cervical deciduous cells and in corresponding cervical biopsies was 87.34%. The sensitivity of immunostaining with p16^INK4A was increased 21.95% for detecting high-grade squamous intraepithelial lesion. Conclusions： The technology of cell block is helpful to diagnose of cervicovaginal cytology. Immunostaining for p16^INK4A on cervical cell block can increase the detective rate and decrease the rate of missed diagnosis for high grade squamous intraepithelial neoplasia and squamous cell carcinoma.
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2006年第9期554-556,i0001,共4页
Journal of Practical Obstetrics and Gynecology
