摘要
目的通过对宫颈癌组织中乙酰肝素酶(heparanase,HPA)、细胞核增殖相关抗原Ki-67和微血管密度的检测,探讨HPA在宫颈癌发生发展中的作用。方法采用免疫组化染色(S-P法)检测67例宫颈癌中HPA、Ki-67和CD34的表达,并与13例正常宫颈组织进行对照研究,分析HPA与患者临床特征、Ki-67和CD34之间的关系。结果67例宫颈癌中49例(73%)阳性表达,而正常13例宫颈组织中无一例阳性表达(P=0.000)。HPA与临床分期、淋巴结转移、细胞增殖和血管生成显著正相关(P值分别为0.001、0.012、0.000、0.000)。结论HPA可能在宫颈癌的发展、浸润、转移和血管生成中起重要作用,并与肿瘤细胞的增殖活性有关,可作为临床预测宫颈癌浸润转移的一个重要参考指标和有价值的治疗靶点。
Objective： To explore the expression of heparanase（HPA） in cervical cancer tissues and the relationship between HPA and cell proliferation, angiogenesis and metastasis by examining HPA, Ki-67 and CD34 in cervical staining（S- P） was used to examine the expression of HPA, Ki-67 and CD34 in 67 paraffin - embedded cervical cancer specimens and 13 normal cervical specimens. The difference of HPA expression between the two groups was analysed by t test, the relationship between HPA and the clinicopathological features as well as the expression of Ki-67 and CD34 was analysed by χ^2 test. Results：HPA was not expressed in normal cervical tissues （ P = 0.000）, but highly expressed in cervical cancer tissues （73% ,49/67）. Expression of HPA significantly correlated with FIGO stage of cervical cancer （ P= 0.001）,lymphatic metastasis （ P= 0.012）, cell proliferation （ P = 0.000） and angiogenesis （ P = 0.000）. Conclusions; HPA may play an important role in the development, invasion, metastasis and angiogenesis of cervical cancer, and have the significant relation with tumor cell proliferation. HPA may be a valuable marker of evaluating biological behavior of cervical cancer and an important target of the treatment.
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2006年第3期154-157,i0001,共5页
Journal of Practical Obstetrics and Gynecology
