摘要
目的:探讨脑热清(NRQ)口服液对内毒素性发热家兔的解热作用。方法:复制大肠杆菌内毒素性发热家兔模型,用数字温度计测量家兔的直肠温度,并采用放免法测定血浆内皮素的含量及血清肿瘤坏死因子(TNFα)的含量,用发色底物法测定血浆组织纤溶酶原激活物(t-PA)和组织纤溶酶原激活物抑制剂(PAI)的活性,同时取肺、肾、肝组织,观察形态改变。结果:①NRQ对内毒素性发热家兔有显著的解热效应(P<0.01);②NRQ可明显抑制发热家兔内皮素、TNFα的含量上升(P<0.01),同时可降低PAI活性(P<0.01)、提高t-PA的活性(P<0.01);③光镜显示,NRQ可减轻发热家兔肺、肾、肝血管扩张充血、淤血病理形态的改变。结论:NRQ可能通过调节发热家兔促凝和抗凝物质的平衡、抑制致热性细胞因子释放达到解热作用。
AIM ： To observe the antipyretic effect of Naoreqing （NRQ） oral solution in rabbits with endotoxin - induced fever. METHODS： The ET- induced fever model was established in rabbits. The digital thermometer was used to register rectal temperature. The endothelin content in blood plasma, and tumor necrosis factor - ot （TNFot） in blood serum were determined by radioimmunoassay. The activities of tissue - type plasminogen activator （ t - PA） and plasminogen activator inhibitor （PAI） in plasma were measured by the assay of chromogenic substrate. At the same time, the pathology of lung, kidney and liver were observed. RESULTS： ① NRQ showed obvious antipyretic effect in the rabbits with endotoxininduced fever （P 〈0. 01 ）. ② NRQ inhibited the endothelin content in plasma and TNFα content in serum, and at the same time reduced activity of PAI （ P 〈0. 01 ） and improved activity of t - PA in plasma （ P 〈0. 01 ）. ③ NRQ reduced the pathology of congestion of lungs, liver and renal in febrile rabbits. CONCLUSION： NRQ has an antipyretic effect . The mechanism is maybe related to its adjusting the balance of coagulant substance and anticoagulant substance, and at the same time inhibiting the secretion of TNFα in febrilic rabbits.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第9期1816-1819,共4页
Chinese Journal of Pathophysiology
基金
北京市科委课题资助(No. 954024200)
关键词
内毒素类
发热
家兔
中草药
Endotoxins
Fever
Rabbits
Drugs, Chinese herbal
