摘要
目的:观察干扰素-α(IFN-α)对人胃癌细胞株BGC-823生长和浸润转移的抑制作用及其作用机理。方法:在体外培养体系中检测IFN-α对BGC-823细胞株生长增殖能力的影响,MTT法检测细胞活力,流式细胞仪检测细胞周期。同时采用免疫组化法观察了IFN-α对肿瘤细胞E-cadherin、MMP-2表达的调节作用,电镜下观察肿瘤细胞间连接的超微结构的变化。结果:IFN-α对人胃癌细胞株BGC-823的生长有明显抑制作用,并表现出剂量依赖性,IFN-α浓度≥106U/L时可有效抑制细胞增殖,抑制率≥12.2%,细胞周期在G1-S期之间发生阻滞;在IFN-α诱导下细胞E-cadherin表达水平升高而MMP-2表达下降,超微结构出现了细胞粘附连接数量增多、结构相对紧密等变化。结论:IFN-α可通过影响细胞周期来抑制人胃癌细胞株BGC-823的生长;IFN-α能调节E-cadherin、MMP-2的表达,密切细胞间的连接,具有限制胃癌细胞侵袭转移的潜能。
AIM ： To observe the inhibitory effect of interferon-α （ IFN-α） on the growth invasiveness and metastasis of human gastric carcinoma cell line BGC - 823, and mechanism of its action. METHODS： We detected the influence of IFN-α on the proliferative ability of BGC -823 in cell culture system, the cell vitality with the MTr colorimetric assay, and the cell cycle with flow cytometer （ FCM）. The regulatory functions of IFN-α to the expression of E - cadherin and matrix metalloproteinase -2 （ MMP -2） in tumor cells were estimated by immunohistochemical analysis （S - P）. The uhrastructural changes of the junction among the tumor cells were observed under electron microscope. RESULTS： IFN-α can significantly inhibit the growth of human gastric carcinoma cell line BGC -823 in a dose -dependent manner. When the concentration of IFN -α was ≥ 10^6 U/L, the cell proliferation can be effectively suppressed,the suppression rate was ≥ 12.2% , and the blockage appeared at the phase of G1 -S of the cell cycle. Under the induction of IFN -α, the expression level of the cell E - cadherin increased while the MMP - 2 decreased. The changes on uhrastructure of the cells showed the increased adhesive junctions and the relative compact structure. CONCLUSION： IFN-α can suppress the growth of human gastric carcinoma cell line BGC - 823 through its influence on cell cycle. IFN-α can regulate the expression of E -cadherin and MMP -2, make the cell junction closely, so that it has the potential on restricting the invasion and metastasis of gastric carcinoma cells.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第9期1756-1761,共6页
Chinese Journal of Pathophysiology
基金
Supported by the Foundation of the Enducation Department of Gansu Province (No. 043B -03)
