摘要
目的探讨病理诊断区域淋巴结阴性（pN0）大肠癌根治切除后早期复发与淋巴结微转移的相关性。方法应用逆转录聚合酶链反应技术，对32例pN0大肠癌患者192枚根治术后病理诊断阴性区域淋巴结的细胞角蛋白20（CK-20）mRNA进行检测，诊断淋巴结微转移。术后1年内对患者进行复查，比较pN0淋巴结CK20mRNA表达与否肿瘤患者复发率的差异。结果13例患者（40．6％）29枚（15．1％）淋巴结检测到CK20mRNA的表达，诊断为淋巴结微转移，TNM分期由I～Ⅱ期上调为Ⅲ期；9例（28．1％）患者肿瘤复发，其中淋巴结微转移7例，无转移者2例，两者比较差异有显著性（校正X^2=5．7，P〈0．05）。结论pN0期患者手术后肿瘤的早期复发与淋巴结微转移有关。
Objective To explore the correlation between early postoperative tumor relapse with lymph node micrometastasis in the patients with pN0 colorectal cancer. Methods Using reverse transcription-polymerase chain reaction （RT-PCR）, 192 regional lymph nodes obtained from 32 patients with colorectal cancer without invasion of the tumor confirmed by histopatbologic examination（pN0 ）were studied for further detecting mRNA of cytokeratin 20 （CK-20） gene and determining nodal micrometastasis. All the patients bad undergone radical resection and regional lymph node dissection and followed up for one year to detect early tumor relapse. Difference in relapse was compared by X^2 test. Results CK20 mRNA expression was i- dentified in 29 lymph nodes （ 15.1% ） ,of 13 patients （40.6%） who were diagnosed as having nodal micrometastasis. TNM staging for these patients was up-regulated from stages Ⅰ -Ⅱ to stages Ⅲ. Relapse disease was found in 9 （28.1% ）patients, 7 of whom had lymph node micrometastasis and 2 without nodal micrometsatasis. Comparison of relapse rate between patients with and without nodal micrometastasis showed significant difference（P 〈 0.05 ）. Conclusion Early tumor relapse after radical surgery in the patients with pN0 colorectal cancer might be correlated with nodal micrometastasis.
出处
《中国肿瘤临床与康复》
2006年第4期295-297,共3页
Chinese Journal of Clinical Oncology and Rehabilitation
关键词
结直肠肿瘤/外科学
淋巴转移
复发
Colorectal neoplasms/surgery
Lymphatic metastasis
Recurrence
