摘要
小鼠每周注射１次空弯菌ＨＳＰ４３共１０ｗ。ＨＳＰ４３抗体第４周起显著升高─直持续到１６ｗ，抗ｄｓ－ＤＮＡ、ｓｓ－ＤＮＡ自身抗体在６ｗ和１６ｗ出现两个高峰，抗ＤＮＡ自身抗体在４～１６ｗ逐渐升高，抗组蛋白抗体在８ｗ最高然后下降，单个小鼠自身抗体反应格局呈现４种类型。各类自身抗体的出现和升高与注射ＨＳＰ４３剂量有依赖关系。该结果为自身免疫病的热休克蛋白发病假设提供了直接的实验证据。
Balb/c mice(8-week-old,female)were immunized with purified 43 kD heat shock protein(HSP43)of Campylobacter jejuni 20,100,200 μg/(time·mouse),i.p. once a week for 10 weeks. Antibody against HSP4 3 and autoantibodies against double strained DNA (dsDNA),single strained DNA(ss-DNA),ENA,and histons were detected by ELISA method.Had found that serological changes in Balb/c mice immunized with HSP43 were similar to the counterparts in the SLE-like animal model induced by chronic infection or immunization with CJ-S131 and in the spontaneous lupus mice,summarized as ① autoantibody against dsDNA started to go up in the 4th week,and peaked in the 6th week,the second peak in the 16th week;② autoantibodies against ss-DNA and ENA went up gradually,the peak responses were achieved in the 16th week;③ peaked in 8th week,the kinetic of autoantibody against histons was different from those of above three autoantibodis;④positive percentages of mice with elevated autoantibodies against ds-DNA,ss-DNA and ENA were higher than that of autoantibody against histons in the 4th,6th,16th week,but not in the 8th week;⑤no autoantibody against RNA was detected;⑥isotypes of autoantibodies were IgG and IgM;⑦the rising of those autoantibodies was the result of injection of HSP43 and relevant to the dosage of injection. The results suggested that specific antigen might not be needed after autoreactive lymphatic clones were selected and activated to some degree,This study provided an experimental evidence for the hypothesis that immune response against HSP may be highly cross-reactive and even involve autoimmune diseases.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1996年第5期279-282,共4页
Chinese Journal of Immunology
基金
国家自然科学基金
卫生部资助
