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IL-18对炎症性肠病患者外周血和肠黏膜固有层淋巴细胞增殖和炎症介质分泌的影响 被引量：2

Effect of IL-18 on peripheral blood and lamina propria lymphocyte proliferation and proinflammatory cytokine secretion in patients with inflammatory bowel disease
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摘要 目的:分析IL-18对炎症性肠病患者外周血和肠黏膜固有层T淋巴细胞增殖和促炎症细胞因子分泌的影响。方法:收集12例克罗恩病(CD)、25例溃疡性结肠炎(UC)患者和15例结肠镜检查正常者(对照)的肠黏膜组织,分离外周血单个核细胞(PBMC)和肠黏膜固有层单个核细胞(LPMC),体外培养并用IL-18刺激,48h后,利用ELISA检测上清液中干扰素(IFN)-γ、IL-2、IL-4、IL-5和肿瘤坏死因子(TNF)-α水平,采用3H-胸腺嘧啶掺入法检测LPMC的增殖情况。结果:IL-18刺激后,CD患者PBMC和LPMCIFN-γ、IL-2和TNF-α分泌水平高于UC和对照(P<0.05);CD患者的LPMC增殖率亦高于UC和健康对照。结论:IL-18可激活CD患者淋巴细胞的增殖,PBMC和LPMC分泌大量促炎症细胞因子,可能参与了CD的发病过程,体内阻断IL-18可能对CD患者免疫治疗有帮助。 Aim ： To investigate the effects of IL-18 on proliferation and proinflammatory cytokine secretion of peripheral blood mononuclear cells （PBMC） and intestinal mucosal lamina propria mononuclear cells （LPMC） from patients with inflammatory bowel disease. Methods：PBMC and LPMC, isolated from 12 patients with Crohn＇s disease, 25 patients with ulcerative colitis and 15 healthy controls, were cultured and stimulated with anti-CD3 and IL-18, and supernatant was harvested after 48 h culture to determine interferon （ IFN）-γ, IL-2, IL-4, IL-5 and tumor necrosis factor （TNF） -α using ELISA. Proliferative response of LPMC was also determined by 3H-thymidine incorporation after 60 h stimulation with IL- 18. Results： PBMC and LPMC from patients with Crohn＇s disease, stimulated with anti-CD3 and IL-18, produced marked- ly higher levels of IFN-γ, IL-2 and TNF-α than those from ulcerative colitis patients and the controls （ P 〈 0.05 ）. Proliferative response of LPMC from Crohn＇s disease patients significantly increased than those from ulcerative colitis patients and healthy controls. Conclusion： IL-18 could induce lymphocyte proliferation and proinflammatory cytokine secretion in patients with Crohn＇s disease. Blocking IL-18 may be helpful in treating Crohn＇s disease.
作者 刘占举 吴国华 张炳勇 徐刚 崔静 赵治国 唐芙爱 徐志林
机构地区 郑州大学第二附属医院消化内科 郑州大学护理学院 河南省人民医院消化内科
出处 《郑州大学学报（医学版）》 CAS 北大核心 2006年第5期828-830,共3页 Journal of Zhengzhou University(Medical Sciences)
基金 国家自然科学基金资助项目30571751 教育部"新世纪优秀人才支持计划"基金资助项目NCET-05-0609
关键词 白细胞介素-18 淋巴细胞 外周血 淋巴细胞 固有层 炎症细胞因子 炎症性肠病 IL-18 lymphocyte, peripheral blood lymphocyte, lamina propria proinflammatory cytokine inflammatory bowel disease
分类号 R574.62 [医药卫生—消化系统]
  • 相关文献

参考文献10

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二级参考文献21

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共引文献15

同被引文献27

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引证文献2

二级引证文献10

郑州大学学报（医学版）
2006年 第5期

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