摘要
背景:溃疡性结肠炎(UC)的病因和发病机制尚不十分清楚,运用新的检测技术和药物干预对深入研究其病因和发病机制有重要意义。目的:通过检测小鼠实验性结肠炎中P-选择素的变化,探索川芎嗪用于治疗UC的可能性及其作用机制。方法:健康昆明小鼠40只随机分为5组(每组8只):正常对照组、模型组和低浓度、中浓度、高浓度川芎嗪培养给药组(分别予川芎嗪0.5mg/ml、1mg/ml和2mg/ml)。除正常对照组外,其余小鼠均以恶唑酮灌肠造模。恶唑酮灌肠后第3天提取外周血单个核细胞(PBMC);并处死小鼠,提取脾脏单个核细胞(SMC)和肠黏膜固有层单个核细胞(LPMC)进行培养给药,采用荧光定量聚合酶联反应(PCR)检测P-选择素表达量。结果:P-选择素在SMC、LPMC和PBMC中的表达量均表现为模型组、低浓度和高浓度川芎嗪组较正常对照组显著增高(P<0.01);在SMC和LPMC中,中浓度和高浓度川芎嗪组均较模型组显著降低(P<0.05)。结论:P-选择素在恶唑酮诱导的小鼠实验性结肠炎中表达增强,川芎嗪具有抑制P-选择素表达的作用。
Background： The etiopathogenesis of ulcerative colitis （UC） is still unclarified, hence, it is important to use new technology and medicinal intervention for its further study. Aims： Through the detection of P-selectin expression in oxazolone-induced colitis in mice and to investigate the possible therapeutic use of tetramethylpyrazine （TMP） in ulcerative colitis and its mechanism of action. Methods： Forty mice were divided randomly in five group, 8 mice in each group： normal control group, model group, low dose group （TMP 0.5 mg/ml）, moderate dose group （TMP 1 mg/ml） and high dose group （TMP 2 mg/ml）. Except the normal control group, experimental colitis was induced in all mice by oxazolone enema. All mice were sacrificed 3 days later, and peripheral blood mononuclear cells （PBMC）, splenic mononuclear cells （SMC） and intestinal mucosal lamina propria mononuclear cells （LPMC） were isolated and cultured in vitro together with TMP. Expression of P-selectin in SMC, LPMC was determined by fluorescence quantitative polymerase chain reaction （PCR）. Results： In all three types of mononuclear cells, the expression of P-selectin in the model group, low- and high-dose TMP groups was significantly higher than that in normal control group （P〈0.01）. In SMC and LPMC, the expression of P-selectin in moderate- and high-dose TMP groups was lower than that in model group （P〈0.05）. Conclusions： The results indicate that expression of P-selectin was increased in oxazolone-induced colitis and can be inhibited by TMP.
出处
《胃肠病学》
2006年第4期219-221,共3页
Chinese Journal of Gastroenterology
基金
上海市自然科学基金项目(No.05ZR14085)资助
关键词
P选择素
川芎嗪
结肠炎
聚合酶联反应
P-Selectin
Tetramethylpyrazine
Colitis
Polymerase Chain Reaction
