摘要
诺卡氏菌形放线菌(Nocardioform actinomycetes)NA3-540产生的β-甘露聚糖酶(ManNA)能不同程度地水解槐豆胶、瓜胶、田菁胶和魔芋胶等甘露多聚糖为组分的植物胶,生成系列甘露寡糖;该酶只轻微地水解香豆胶,不能水解β-甘露聚糖、黄原胶、海藻胶;ManNA对槐豆胶、瓜胶和魔芋胶多糖的Km值和Vmax分别为1·75、6·13、3·9mg/mL和2485、1303、853μmol/(min/mg),表明槐豆胶是该酶的理想水解底物。ManNA水解几种植物胶的明显差异,表明甘露聚糖的糖链组成和空间结构明显地影响着β-甘露聚糖酶的水解活性。
Endo-1, 4-β-D-mannanase （ManNA） from.a Nocardioform actinomycetes NA3-540 was capable of hydrolyzing locust bean gum, guar gum, tian-jing gum, konjak gum and cassia tora gum to product serial manno-oligosaccharides in different velocity and degree, but it hydrolyzed the cessiafora gum in a slight limit and hardly hydrolyzedα-mannan, xanthan, alginates and carrageenan. Michaelist constants （K.） of the ManNA for locust bean gum, guar bean gum and konjak gum were 1.75, 6. 13,3.9 mg/mL, and maximum velocity （ Vmax ） of the enzyme for these saccharides were 2485, 1303, 853μmol/ （min/mg） respectively. It suggested that Locust bean gum was the better hydrolysis substrate for the enzyme. It was observed that there were significant different for the ManNA in the hydrolysis of several mananan gums. It suggested that the hydrolysis progress was affected by the composition and structure of these polysaccharides.
出处
《微生物学通报》
CAS
CSCD
北大核心
2006年第4期31-35,共5页
Microbiology China
基金
中国科学院知识创新工程重要方向项目(No.KSCX2-SW-323)
