摘要
利用酶联免疫测定技术,研究了萝卜金花薹细胞质雄性不育系75A及其保持系75B幼根、叶和花蕾中内源激素IAA、GA、ABA和ZR含量的动态变化。结果表明:75A和75B的根、叶和花蕾发育过程中激素含量变化差异明显。在花蕾各个发育时期,75A和75B的IAA、ABA和GA含量均为先降后升,75A花蕾中IAA含量低于75B,ABA和GA的含量均高于75B;75A的ZR含量动态变化先升后降,与75B相反。在营养器官中,75A的根和叶中ZR、GA和ABA的含量高于75B(除个别时期外),IAA含量则是75A低于75B。分析认为根、叶和花蕾中内源激素的异常变化可能与萝卜小孢子败育有关。
Content changes of IAA, GA, ABA and ZR were determined with enzyme-linked immunosorbent assay （ELISA） method in roots, leaves and buds of Jinhuatai cytoplasmic male sterile （CMS） radish 75A and its maintainer 75B during different development stages. The results indicated CMS 75A and its maintainer line 75B showed significant differences in their hormone content in seeding root, leaves and buds. Changes in IAA, ABA and GA content in different development stages of buds of 75A and 75B had a similar pattern, i.e. in both lines, the levels of IAA,GA and ABA dropped at first, then rose, however, IAA content of buds in 75A was lower than that in 75B, ABA and GA contents were on the contrary. ZR content in buds of CMS rose in the early stage and then cut down, and its maintainer line had an opposite pattern. The ZR, ABA and GA content of seeding roots and leaves in 75A were higher than that in75B except exceptional stages, while IAA content was on the contrary. All analyses suggest the abnormal hormone change may be related to the microspore abortion in root, leaves and buds of male sterile radish.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2006年第4期407-410,414,共5页
Journal of Jilin Agricultural University
基金
辽宁省科技攻关项目(2001215002)
