摘要
目的比较细胞培养和酶联免疫法(EIA)检测沙眼衣原体的效果。方法收集性病门诊患者标本1 350例分别进行细胞培养和EIA法检测,另将两法检测结果不相符的标本采用荧光PCR复检,比较分析细胞培养,EIA法与综合结果的相符性。结果分析表明细胞培养与EIA法检测沙眼衣原体的敏感性分别为65.77%和93.96%,特异性分别为100%和99.0%。结论EIA法检测沙眼衣原体有较高的敏感性而且方法简便、快速,可以用于大规模标本的检测,是世界目前实验室最普遍的衣原体筛查方法。
Objective To analyze the significance of detection of cell culture and EIA in diagnosis of Chlamydia trachoma from samples of outpatients with venereal disease. Methods 1 350 samples were collected from outpatients wth venereal disease and cultured, then determined by using EIA. The samples that were inconsistent with two detection methods would be reexamined by fluorescence PCR. The results were compared. Results Rates of sensitivity of ceil culture and EIA were 65.77% and 93.96% ; the rates of specificity were 100% and 99.0%. Conclusion EIA is a sensitive, convenient and fast method for detection of Chlamydia trachoma infection, suitable for massive screening specimens.
出处
《中国热带医学》
CAS
2006年第8期1471-1472,共2页
China Tropical Medicine
