摘要
目的 :评价MicrotrakⅡEIA法用于女性宫颈拭子、男性尿道拭子和尿沉渣标本沙眼衣原体检测效果。方法 :采用细胞培养法作为“金标准”方法 ,PCR为辅助标准 ,MicrotrakⅡEIA法平行测定。结果 :共检测 1 64例标本 ,以细胞培养法作金标准 ,总体MicrotrakⅡEIA检出率高于细胞培养法 ( χ2 =5 0 0 ,P <0 0 5 ) ;对于女性宫颈和男性尿沉渣标本 ,MicrotrakⅡEIA法与培养法的检出率无显著性差异( χ2 =1 0 0和 χ2 =0 2 0 ,P均 >0 0 5 ) ,而男性尿道拭子标本的MicrotrakⅡEIA法检出率高于培养法 ,差异有统计学意义 ( χ2 =4 0 0 ,P <0 0 5 )。以细胞培养法加PCR法作标准 ,检测各样本的结果差异无统计学意义。结论 :MicrotrakⅡEIA法具有较高的敏感性和特异性 ,适合于泌尿生殖道沙眼衣原体感染的实验室诊断。
Objective:To Evaluate Microtrak Ⅱ EIA for detecting Chlamydia trachomatis from female cervical and male urethral swabs and sediments of male urine.Methods:Cell culture was adopted as “golden standard”, PCR as a supplement and Microtrak Ⅱ EIA were used as parallel to detect Chlamydia trachomatis. Results:164 samples were detected. Compared with cell culture method, the whole sensitivity of Microtrak Ⅱ EIA were showed no statistics difference for female cervical swabs and male urine sediments, while Microtrak Ⅱ EIA was more sensitive than cell culture for male urethral swabs(χ~2=4.00,P<0.05). Cell culture plus PCR as a standard, no statistics difference between Microtrak Ⅱ EIA and the standard for all kinds of samples in terms of sensitivity was observed. Conclusion:It was suggested that Microtrak Ⅱ EIA had high sensitivity and specificity, it could be used for laboratory diagnosis of Chlamydia trachomatis infection in genitourinary tract.[
出处
《岭南皮肤性病科杂志》
2004年第3期207-209,共3页
Southern China Journal of Dermato-Venereology
