精神应激Balb/c小鼠不同部位脑组织中IL-1β及IL-6水平的变化被引量：3

Cerebral IL-1β and IL-6 levels in a psychological stressed Balb/c mice model

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摘要目的探讨精神应激(天敌应激)小鼠模型皮层、海马及下丘脑IL-1β,IL-6水平的变化,深入了解精神应激对脑组织中细胞因子的影响。方法随机将Balb/c小鼠分别暴露在正常环境(对照组)、急性(45min,1次)、慢性(45min/次,1次/d,连续14d)鼠类天敌(猫)应激环境中。应激结束后用EIA法检测血浆皮质酮水平,用ELISA法测定皮层、海马及下丘脑匀浆中IL-1β,IL-6的含量。结果①血浆皮质酮:应激后水平升高,以慢性应激组显著,与正常生理组比较差异有显著性(P<0.05);②皮层IL-1β,IL-6水平:急、慢性应激组水平均降低,以慢性应激组更显著,与正常对照及急性应激组差异均有显著性(P<0.05)。③海马IL-1β,IL-6水平:急、慢性天敌应激后IL-1β水平降低,应激组与正常生理组比差异均具有显著性(P<0.01);IL-6水平无明显变化(P>0.05)。④下丘脑IL-1β,IL-6水平:急性应激组IL-1β水平降低而慢性应激组水平略升高,但变化均差异无显著性(P>0.05);应激组IL-6水平均降低,以急性应激变化更为明显,其与正常对照组及慢性应激组相比差异有显著性(P<0.05)。结论精神应激可影响小鼠脑组织中细胞因子的产生,细胞因子的变化在精神应激导致功能紊乱的过程中有重要作用。 Objective To investigate IL-1β and IL- 6 levels in cortex, hippocampus and hypothalamus in a psychologically stressed mice model （cat -exposed predatory stress）, thus to further demonstrate the responses of neuro - immuno -encocrine network when subjected to psychological environments. Methods Mice were divided into 3 groups, normal control, acute and chronic stress group. Stressed mice, ie cat - exposed mice received either a single 45 - min cat exposure （ acute stress group） or daily 45 - min exposure to the cat for consecutive 14 days （ chronic stress group）. There- after, plasma corticosterone （CS） was detected by EIA and homogenization of cortex, hippocampus and hypothalamus was used for detection of IL -1β and IL - 6 by ELISA. Results （1）Plasma CS levels ： plasma CS was elevated in predatory stress mice, particularly in chronic stress group. There was a significant difference compared to normal control （P 〈 0.05）. （2）IL -1β and IL - 6 levels in cortex ： under predatory stress, both IL -1β and IL - 6 levels decreased and the chronic stress mice had the lowest level. A significant difference was revealed if compared with the normal and acute stress group （P 〈 0.05）. （3）IL -1β and IL - 6 levels in hippocampus ： IL -1β level decreased in both cat - exposed groups, and if compared with control group, there were significant differences between normal and stressed groups （P 〈 0.01 ）. IL -6 elevation in stressed group showed no significant differences compared to control group （P 〉 0.05 ）. （4） IL -1β and IL - 6 levels in hypothalamus ： for IL -1β, there was mild elevation in chronic stress group while decrease in acute group. Significant differences were not found between normal and stressed mice （ P 〉 0.05 ）. IL - 6 level exhibited decrease in both acute and chronic groups, with more prominent decreased in acute group. Furthermore, there were significant differ- ences if compared with normal group （P 〈 0.05）. Conclusion Psychological stress （ cat - exposure predatory stress） in- fluences the secretion of cerebral cytokines, which engages a neurocircuitry and multiple molecules that may fuel long - standing changes in brain response to stress.

作者苏芸谢泽锋李康生

机构地区汕头大学医学院微生物学与免疫学教研室汕头大学第一附属医院

出处《广东医学》 CAS CSCD 北大核心 2008年第9期1446-1448,共3页 Guangdong Medical Journal

基金国家自然科学基金资助项目(编号:30271211 30371315)

关键词精神应激天敌脑细胞因子 Psychological stress Predatory Brain Cytokines

分类号 R392 [医药卫生—免疫学] R57 [医药卫生—消化系统]

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