摘要
利用微卫星(Microsatellite)分子标记对我国16份披碱草Elymus dahuricus进行遗传多样性研究。研究共筛选了38对引物,其中18对引物有扩增条带,占总数的47.4%,具有多态性并且扩增效果较好的引物有6对,占总数的15.8%。用筛选出的这6对引物对供试的16份材料进行微卫星分析,6对引物共检测出26个等位基因,平均每对引物所产生的平均等位基因数为4.3个位点;利用微卫星分子标记数据,对其居群遗传分化进行研究,结果表明:披碱草77.36%的遗传变异出现在居群内,因此,披碱草的遗传多样性主要存在于居群内;对16份披碱草进行聚类分析,构建了亲缘关系树状图,16份供试材料大致分为5类。
Genetic diversity of 16 accessions were studied at microsatellite level. Thirty-eight primer pairs were used for polymorphic selection,with six （15.8%） primer pairs produced polymorphic products. A total of twenty-six bands amplified from six primer pairs were selected for microsatellite analysis. Primers consistently indicated that 77.36% of the variation existed within the groups of the 16 accessions of Elymus dahuricus; By cluster analysis with sum of squares for microsatellite showed that 16 accessions can be divided into 5 groups with different characteristics,which could afford refer- ence in deeper research of E. dahuricus.
出处
《草业科学》
CAS
CSCD
2006年第8期40-44,共5页
Pratacultural Science
基金
国家科技部基础研究专项基金(2003-2005)
