摘要
本文研究了蛋白水解酶引导鱼蛋白水解物的类蛋白反应。Sephadex G-25凝胶层析证明,蛋白水解酶确实可以引导鱼蛋白水解物中的小分子多肽等发生类蛋白合成反应。酶引导的类蛋白合成反应的最适条件:对胰蛋白酶和糜蛋白酶最适pH是7,最适保温时间为24小时,最适底物浓度40％,最适酶与底物比为1:(?)00在最适条件下,胰蛋白酶可以得到26.52％,糜蛋白酶可以得到20.78％收率的类蛋白。
The plastein reaction that enzyme induced was studied. Fish protein hydrolysates were employed for plastein synthesis. Enzyme induced plastein reaction was optimized at pH 7 for trypsin and chymotrypsin; substrate concentration, 40% (w/v); time of incubation, 24hr; enzyme substrate ratio, 1:100(w/w), Percent Plastein yields of 26.52% and 20.78% was obtained for trypsin and chymotrypsin, respectively, when trichloracetic acid was used as the precipitating agent.
出处
《大连水产学院学报》
CSCD
1990年第1期9-16,共8页
Journal of Dalian Fisheries University
关键词
蛋白水解物
类蛋白
酶
protein hydrolysate, plastein., enzyme
