摘要
从土壤中分离得到一株放线菌菌株,杯碟法测定该菌株发酵液对14种植物病原真菌和两种植物病原细菌均有不同程度的抑菌活性,其中对烟草黑胫病菌和黄瓜菌核病菌的抑菌效果最好,抑菌圈直径达56.36mm和56.26mm。枯斑抑制法测定发酵液对TMV的抑制作用表明,发酵液对TMV有很好的钝化作用,发酵液与病毒汁液混合10min后接种,枯斑抑制率为93.88%,接种前24h处理和接种后24h处理的枯斑抑制率分别为87.93%和42.21%。通过对发酵液稳定性的初步研究,表明发酵液中的抗菌物质对温度、光照以及pH值变化都比较稳定。
Actinomycete mycelia were isolated from soil and a cylinder-plate assay was used to determine that fermentation broth from these fungal mycelia had varying degrees of inhibitory activity towards 14 plant pathogenic fungal species and 2 plant pathogenic bacterial species. Among these, activity against Phytophthora parasitica var. nicotianae and Sclerotinia sclerotiorum was the highest, with inhibition diameters of 56.4 and 56.3 mm, respectively. A necrotic spot method was used to determine that the fermentation broth inactivated TMV. When inoculating 10 rain after mixing fermentation broth with viral sap, necrotic spot formation was inhibited 93.88%. Treatment 24 h before or after inoculation provided 87.93 and 42.21% inhibition, respectively, of necrotic spot formation. Preliminary studies were conducted on stability of the fermentation broth. The antimicrobial substance in the fermentation broth was stable to temperature, light, and pH.
出处
《农药》
CAS
北大核心
2006年第8期525-526,528,共3页
Agrochemicals
基金
国家烟草专卖局科技项目(项目号:国烟科[2002]187号)
辽宁省科技攻关项目(2001208001)
关键词
放线菌
发酵液
抗菌谱
稳定性
Actinomycetes
fermentation broth
antimicrobial spectrum
stability
