摘要
用活细胞染色体ＤＮＡ可与外源性ＤＮＡ同源序列发生同源重组的性质，达到定点修饰改造染色体某基因的目的，此法称基因打靶．基因的同源重组是较普遍的生物现象，其分子机理尚未阐明，但活细胞内确有一酶系可使ＤＮＡ的同源序列在细胞内发生重组，这一事实已无可争辨．此事实为基因打靶的理论基础．基因打靶技术操作的关键是建立一含筛选基因的重组载体，并有效地把它转入细胞核内．基因打靶命中的细胞可稳定遗传．基因打靶在改造生物品种，一些复杂生命现象（如发育的分子机制等）及临床理论研究均有广阔的前景．
In living cells;exploiting homologousrecombination between sequences both fromgenomic DNA and extracellular DNA toaccomplish site-modification in a certain gene onchromosome;is a method called gene targeting.Although the molecular mechanism of DNAhomologous recombination is far from being elucidated; it is a common biological phenomenon.It is true that in cells there is an enzyme systemthat plays the role to make recombination ofhomologous DNAs; which is the theoreticalfoundation of gene targeting. The technology ofgene targeting has been fully developed.The keystep for operation is to construct a recombinantcarrier including one or two genes for selectionand to transfer it into the nucleus effectively.The targeted cells can be inherited stably.Genetargeting has promising prospects in productionof new strains of living things;in clinical usageand in theoretical research of some complicatedbiological phenomena such as the molecularmechanism of development.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1996年第6期500-505,共6页
Progress In Biochemistry and Biophysics
关键词
基因打靶
基因剔除
DNA同源重组
gene targeting
gene knocking out
homologous recombination
