摘要
甲基磺酸乙酯(EMS)是一种稳定、高效的诱发点突变的诱变剂。采用EMS处理大豆"南农94-16"的种子,在初步构建大豆突变体库的基础上进一步扩建了大豆突变体库,获得95份茎、叶、花、种子等性状变异的突变材料。采用200对SSR标记分别对其中高蛋白、叶黄化等9个不同性状的突变体进行了鉴定。结果表明:与对照相比突变体发生了多位点的变异。构建的突变体库不但可以作为新的种质资源,而且可促进大豆功能基因组研究的开展。
EMS is a stable and effective mutagen. In this study, soybean mutant pools were constructed by treating the seeds of soybean＂ Nannong 94-16＂ with EMS. These pools include 95 mutants of stem, leaf, cotyledon,flower and seed. In addition,200 SSR primer pairs were used to characterize several mutants such as high-protein content mutant,revealing polymorphism between mutants and base materials. These mutant pools created for＂ Nannong 94-16＂ will be valuable for the research in breeding and functional genomics soybean.
出处
《大豆科学》
CAS
CSCD
北大核心
2009年第4期574-577,582,共5页
Soybean Science
基金
国家重点基础研究发展计划(973计划)资助项目(2004CB117206)
国家高技术研究发展计划(863计划)资助项目(2006AA10Z1C1)
