摘要
用膜片钳连细胞电压钳法，在培养的Ｗｉｓｔａｒ大鼠单个心肌细胞上记录了Ｔ－２毒素对Ｂ、Ｌ和Ｔ三型Ｃａ２＋通道单通道电活动的影响．结果表明，Ｔ－２毒素浓度为１０ｍｇ／Ｌ时，心肌细胞Ｂ、Ｌ和Ｔ三型Ｃａ２＋通道均受到明显的阻滞，其阻滞作用表现为使Ｃａ２＋通道的开放概率减小，开放时间缩短，关闭时间延长，而对流过Ｃａ２＋通道的Ｂａ２＋流幅值无影响．
The effects of T-2 toxin on the activities of B-type, L-type and T-type Ca2+ channelswere recorded on cultured ventricular myocardiocytes of neonatal Wistar rats by cell-attachedpatch clamp technique. T-2 toxin at dose of10 mg/L significantly inhibited the activities ofthe three types of Ca2 + channels. The open timeof Ca2+ channels was shortened and the openstate probability was decreased, whereas therewere no significant variations in the amplitude ofionic current flowing through the Ca2 + channels.Compared with verapamil and Bay K 8644, theeffects of T-2 toxin on the Ca2+ channels weresimilar to the blockade effect of verapamil. Itwas suggested that the cytotoxic action of T-2toxin may be due to the damages of cellmembrane.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1996年第5期445-449,共5页
Progress In Biochemistry and Biophysics
关键词
T-2毒素
心肌细胞
钙通道
阻滞作用
毒理学
fusarium toxin, T-2 toxin, cardiac cell, calcium channel, patch clamp technique
