摘要
为探讨成纤维细胞中Ｃａ２＋的主要来源及其对胶原合成的作用，观察了钙通道阻滞剂桂利嗪和具有胞内Ｃａ２＋拮抗作用的尼卡地平对３Ｈ－Ｌ－脯氨酸参入人成纤维细胞的影响。桂利嗪抑制胶原合成的作用呈浓度依赖性，ＩＤ５０为４１．２μｍｏｌ·Ｌ－１。尼卡地平作用２４ｈ表现为浓度依赖性抑制作用，但在３６，４８和６０ｈ低浓度组（１０，２０μｍｏｌ·Ｌ－１）外，高浓度组（４０，８０μｍｏｌ·Ｌ－１）的抑制作用降低。
To determine the major origin of intracellular calcium ion(Ca2+) and the effect of intracellular Ca2+ on the collagen synthesis of human lung fibroblasts, we observed the influence of cinnarizine(Cin)and nicardipine(Nic),an intracellular calcium antagonist, on the incorporation of labeled 3H-proline. The results showed that Cin decreased the collagen synthesis and this effect was concentration dependent (ID50=41. 2μmol·L-1). The collagen synthesis was also suppressed in a drug concentration-dependent manner after 24 hours of exposure to Nic(10 to 80μmol·L-1),and this suppressIon disappeared after 36 to 60 hours of continuous exposure to high concentrations of Nic(40, 80μmol·L-1), but not to low concentrations(10,20μmol·L-1).
出处
《中国药理学通报》
CAS
CSCD
北大核心
1994年第4期308-310,共3页
Chinese Pharmacological Bulletin
关键词
桂利嗪
尼卡地平
胶原合成
cinnarizine
nicardipine
collagen synthesis
