摘要
为研究甲型肝炎减毒活疫苗（L－A－1株）口服免疫的免疫原性，114只小白鼠1次口服免疫后1～8周内，每周收集肠洗液和血清样品，用ELISA方法检测特异抗体反应。111只小白鼠检测到阳性抗体反应，阳转率为93．37％。肠洗液S－IgA阳转率高峰在第1和第6周（65％和64％）。血清IgA于第一周未测到，其高峰在第6周（100％）。IgM于第1周即达高峰（88％），IgG于第3周达高峰（88％）。至第8周末，S－IgA维持在40％，IgA、IgM和IgG分别维持在90％，80％和80％。实验结果表明，该疫苗口服免疫后具有良好的免疫原性，可激活小白鼠的局部与全身免疫反应。
In order to study on the immunogenicity of live attenuated hepatitis A vaccine (L-A-1 strain) via oral immunization, the samples of intestinal lavage fluids and serum of 114 mice were collected every week wlthin 1-8 weeks after being immunized and detected for specific antibody response by ELISA. The hepatltis antibody positive conversion rate reached 93. 37% (111/114 ). The peak values of S-IgA positive conversion rate in intestinal lavage fluids were observed at the 1st (65% ) and the 6th (64% )week. Serum IgA was not detected out at the Ist week, however,the positive conversion rate of it reached 100% at the 6th week.The peak value of positive conversion rates of IgM and IgG were observed at the 1st(88% ) and the 3rd (88%) week respectively. By the end of the 8th week, the posltive conversion rates of S-IgA,IgA,IgM and IgG were 40%, 90%, 80% and 80%,respectively. The result showed good immunogenecity of orally administered live attenuated hepatitis vaccine which could successfully stimulate the local and systemic immunosystems of the mice.
出处
《中国生物制品学杂志》
CAS
CSCD
1996年第4期168-170,共3页
Chinese Journal of Biologicals
关键词
甲型肝炎
减毒活疫苗
口服免疫
特异抗体反应
Live attenuated hepatitis A vaccine
Oral immunization
Specific antiblody response
