摘要
目的:观察神经干细胞和脑源性神经营养因子联合应用对老年痴呆大鼠学习记忆能力及其基底前脑小白蛋白神经元的作用。方法:实验于2003-12/2005-10在广州医学院解剖教研室神经生物学实验室完成。取健康雄性SD大鼠40只,随机分为4组:正常对照组、损伤组、移植组和联合组,每组10只。①利用无血清培养技术获得新生SD鼠的海马神经干细胞。②除正常组外,其他3组切断SD大鼠左侧穹隆海马伞建立老年痴呆大鼠模型;移植组和联合组,基底前脑注射神经干细胞;联合组同时侧脑室注射脑源性神经营养因子。③4周后行Y迷宫测试大鼠学习、记忆能力(学习次数分值高表示差,记忆能力分值低表示差),免疫组织化学结合图象分析技术观察各组大鼠基底前脑小白蛋白阳性神经元数目受影响的情况。结果:①损伤组大鼠比正常对照组大鼠学习、记忆能力明显下降(98.2±5.6比59.8±4.1,3.5±0.5比8.4±0.4,P<0.01)。②移植组学习、记忆能力均有改善(74.1±5.4,6.7±0.5,P<0.05),但与正常组比较差异有显著性意义(P<0.05)。③联合组学习记忆能力(56.7±0.9,8.3±0.3)与正常组比较无显著性差异(P>0.05)。④大鼠的学习记忆能力与基底前脑小白蛋白阳性细胞数呈正相关。结论:神经干细胞和脑源性神经营养因子联用较单独使用神经干细胞或脑源性神经营养因子更好地改善老年痴呆大鼠的学习记忆能力。
AIM： To observe the effect of Combined application of noural stem cells （NSC） and braln-deprived neurotrophic factor （BDNF） on learning and memory abilities and parvalbumin in basal forebrain of model rats with senile dementia. METHODS： This experiment was conducted at the Laboratory of Nourobiology, Department of Anatomy, Guangzhou Medical College from December 2003 to October 2005. Totally 40 healthy male SD rats were used and randomly divided into 4 groups： normal control group, lesion group, transplantation group, and combined application group with 10 rats in each group. （1） NSCs were obtained from hippocampus with serum-free culture. （2） Except for normal control group, the rats in the other 3 groups undergone left fimbria-fornix（F-F） transection and were established senile dementia rat models; NSCs were injected into the basal forebrain of the rats in the transplantation group and combined application group; BDNFs were also injected into the lateral ventricle of the rats in the combined application group. （3） Learning and memory abilities were measured by Ymaze test after 4 weeks . The number of basal forebrain parvalbumin positive neurons was analyzed by using immunohistochemical method combined with technique of micromeasure and image analysis. RESULTS： （1） Compared with control group, remarkable decrease in spatial learning and memory abilities were found in the injured group （98.2±5.6 vs 59.8±4.1,3.5±0.5 vs 8.4±0.4,P 〈 0.01）. （2）The rats in the transplantation group had significant improvement in learning and memory abilities （74.1±5.4,6.7±0.5,P 〈 0.05）, while significant difference was found between transplantation group and control group（P 〈 0.05）. （3）There were no significant differences in learning and memory abilities between combined application group and control group（P 〉 0.05）. （4）The abilities of learning and memory were positively related to the number of parvalbumin positive cells in the basal forebrain. CONCLUSION： Combined application of BDNF and NSC is more effective in improving the ability of spatial learning and memory in model rats with senile dementia than application of BDNF or NSC alone.
出处
《中国临床康复》
CSCD
北大核心
2006年第25期40-42,共3页
Chinese Journal of Clinical Rehabilitation
基金
广东省自然科学基金(04009566)
广东省医学科研基金(A2003276)
广州市市属高校科技计划重点项目(041011)
广州医学院科研项目(04-K-01)~~
