摘要
背景:神经行为是评价中枢神经发育异常的最敏感指标,导致其发育畸形的影响因素较多。为此,探讨高温与香烟联合环境下对神经行为发育的影响。目的:探讨高温与香烟联合对仔鼠神经行为发育的影响。设计:以动物为观察对象的随机对照实验。单位:新乡医学院第三附属医院妇产科和新乡医学院的细胞生物学教研室。材料:健康未生育的SD大鼠270只,雌性大鼠180只,体质量190～210g,雄性大鼠90只,体质量225～275g,鼠颗粒饲料喂养,常规饮水,自然光照,室温25℃。将动情期的雌雄大鼠按2:1合笼过夜,次日晨检到阴栓为妊娠第0d,共获孕鼠160只,随机分成16组,暴露于高温、香烟及高温联合香烟的条件下,孕鼠自然分娩,观察仔鼠的早期反射和学习记忆。干预:160只孕鼠从妊娠第2d开始,37℃,41℃,42℃高温联合香烟组大鼠每只隔日皮下注射相当于1.0支香烟水溶物,25℃对照组和37℃,41℃,42℃单独高温组每日注射2.0mL的双蒸水,共7次。于孕第10d将孕鼠置于温箱中,37℃,41℃,42℃单独高温组肛温分别保持在(37+0.5)℃,(41+0.5)℃,(42+0.5)℃,持续2.0min;37℃,41℃,42℃高温联合香烟组肛温保持同单独高温组,每组均分别持续0.5,1.0,1.5,2.0min;25℃对照组不做高温处理。受孕18d后单笼饲养待其自然分娩。记录妊娠天数、仔鼠数、死胎数。
BACKGROUND:Neural behavior is a sensitive parameter for predicting abnormal ce ntral nervous growth that is resulted from multiple factors.Therefore this study was designed to explore the influence of hypothermia and cigarette smoke exposu re during pregnancy on neural behavioral growth of neonatal rats. OBJECTIVE:To study the influence of hypothermia and cigarette smoke exposure o n neural behavioral growth of neonatal rats. DESIGN:Randomized case control study based on experimental animals. SETTING:Obstetric and Gynecological Department ,Third Affiliated Hospital of X inxiang Medical College;Cytobiological Department of Xinxiang Medical College. MATERIALS:Totally 270 healthy unpregnant SD rats were obtained,including 180 m ales with body mass of 190-210 g,and 90 female rats with body mass of 225-275 g.Rats were raised with granulated feed and drinking water routinely in room of 25 °C with natural sunlight.Male and female rats in estrus were put in the same cage overnight in proportion of 2:1,pregnancy was confirmed if vaginal bolt wa s observed the following morning.Totally 160 pregnant rats were obtained and ran domly divided into 16 groups that were exposed to hyperthermia,cigarette smoke o r their combination and by this way, earlier reflex,learning and memory of neona tal rats were observed after natural delivery, INTERVENTIONS:From the second day of pregnancy, rats in 37 ℃,41 ℃,42 ℃hyper thermia combining cigarette group received subcutaneous injection of 1 mL smoke hydrotrope every other day, while rats in 25 ℃control group and 37 ℃, 41 ℃,42 ℃single hyperthemia group were given 2.0 mL double distilled water every day f or totally 7 times.From the pregnancy of 10 days, pregnant rats were put into in cubator, and rats anus temperature should be kept respectively at(37±0.5) ℃, ( 41±0.5)℃,(42±0.5)℃in the 37 ℃, 41 ℃,42 ℃of single hyperthemia groups and the temperature should last for 2.0 minutes;rats of 37 ℃, 41 ℃, 42 ℃hyperthem ia combining cigarette group received the same treatment and were divided into f our subgroups with the duration of 0.5, 1.0, 1.5,2.0 minutes respectively;rats i n 25 ℃control group were not exposed to hyperthermia.Rats were then singly rais ed from the pregnancy of 18 days for natural delivery.Pregnant days,number of ne onatal rat and death rats were recorded,and visible malformation was observed.Th e average body masses of each cote of neonatal rats were measured every week.Tim e of eye opening,ear opening,hair growing and teething were observed for asse ssing their physical growth.Reflex and sensory function of neonatal rats,such as planar tuning,spatial turning,visual orientation and auditory surprise was obse rved at 3,7,12,16 days after birth respectively;The mean targeting time of each cote was used as reference for assessing their reflex.Y lybirinth test was used for assessing learning and memory function of neonatal rats(30 days after birth ). MAIN OUTCOME MEASURES:①Main outcomes:Reflex and sensory, such as planar tunin g,spatial turning,visual orientation and auditory surprise.②Secondary outcomes: Influence of hyperthemia and cigarette exposure on learning memory function of n eonatal rats. RESULTS:For hyperthermia group(41°C and 42°C), targeting time of neonatal ra t physical growth(eye opening,ear opening,hair growing and teething) and refl ex sensory function(planar tuning,spatial turning, nvisual orientation and audit ory surprise),as well as learning and memory(minute) were obviously delayed in c ontrast with those of the control group and 37 °C group(χ2=11.34,P< 0.01);mean while,longer hyperthermia exposure (1.5 minutes) combined with cigarette smoke h ad supplementary effect on the above neural behaviors,which was significantly di fferent from that of single hyperthermia group(χ2=10.29,P< 0.01). CONCLUSION:Abnormal neural behavior of neonatal rats, such as growing retardat ion,learning and memory obstacle could be induced by the combination of longer h yperthemia exposure and cigarette smoke.
出处
《中国临床康复》
CSCD
北大核心
2005年第16期251-253,共3页
Chinese Journal of Clinical Rehabilitation
基金
河南省自然科学基金资助项目(97402500)~~
