摘要
目的:研究丙型肝炎患者血清中的丙型肝炎病毒核酸(HCV-RNA)载量与抗-HCV含量以及丙氨酸氨基转移酶(ALT)水平之间的相关性。方法:应用聚合酶链反应(FQ-PCR)技术定量检测231份HCV-RNA临床标本,同时采用ELISA法检测抗-HCV,用生化分析仪测定ALT水平。结果:231份样本中有136份HCV-RNA含量高于80拷贝/ml血清,阳性率为58.9%(136/231);139份样本抗-HCV阳性,阳性率为60.2%(139/231);100份样本ALT异常,异常率为43.3%(100/231)。结论:应用FQ-PCR方法检测HCV-RNA特异性强、灵敏度高,具有良好的临床应用价值。
Objective To explore the relation between the quantity of HCV-RNA and the contents of anti- body to HCV and ALT standard in the serum samples of HCV patients.Methods The quantity of HCV-RNA were determined by FQ-PCR technique,the contents of antibody to HCV by ELISA,ALT standard by biochemistry analysis equipment.Results 136 HCV-RNA of 231 samples were more than 80 copies/ml,the positive rate was 58.9%（136/231 samples） ;139 samples were detected anti-HCV,the positive rate was 60.2%（139/231 samples）; 100 samples were detected,its results were more than 40 u/L,the positive rate was 43.3%（100/231 samples）. Conclusion FQ-PCR is a specific and sensitive method for the detection of HCV infection,and can be widely used in clinical detection.
出处
《安徽卫生职业技术学院学报》
2006年第3期34-35,共2页
Journal of Anhui Health Vocational & Technical College
