摘要
研究了青梅黄酮类化合物的新微乳薄层色谱分离鉴定方法。以调酸后的十二烷基硫酸钠-正丁醇-正庚烷-水微乳液为展开剂,利用聚酰胺薄膜层析色谱法能使青梅黄酮类化合物完全分离。从青梅果皮中分离得到6个黄酮类化合物斑点,果肉中得到3个黄酮类化合物斑点,但二者的斑点显著不同。与用正丁醇-乙酸-水(4∶1∶5)为展开剂的常规层析方法相比,微乳薄膜层析法检测灵敏度更高,分离效果更好。
A new method on separation and identification of flavonoids in plum was studied. Influence of microemulsion type on resolving power toward flavonoids was investigated with sodium dodecyl sulfate/n-C4H9OH/n-C7H16/H2O microemulsion as eluant on polyamide film. The flavonoids were separated entirely. Six flavonoid spots were obtained from fruit peel and three from pulp, but they were not identical. Compared with the general mobile phase composed of C4H9OH/CH3COOH/H2O（4：1：5）, the sensitivity of detection was improved markedly and the results were satisfactory. This new chromatographic method is simple, accurate for separation and identification of flavonoids in plum.
出处
《林产化学与工业》
EI
CAS
CSCD
2006年第2期53-56,共4页
Chemistry and Industry of Forest Products
基金
广东省农业攻关计划项目(2003A2030502)
关键词
青梅
黄酮类化合物
微乳液
薄膜层析
plum
flavonoids
microemulsion
thin-layer chromatography
